Characteristics of enzymatic hydrolysis of thermal-treated wheat gluten |
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Authors: | Jin-shui Wang Zhi-yan Wei Lu Li Ke Bian Mou-ming Zhao |
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Affiliation: | aSchool of Food Science and Technology, Henan University of Technology, Zhengzhou 450052, PR China;bCollege of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China |
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Abstract: | Effect of thermal treatment at 50–90 °C on wheat gluten hydrolysis by papain was evaluated in this study. Thermal treatment decreased the amount of sodium dodecyl sulfate (SDS) extractable protein. The treatments at 80 and 90 °C had a strong impact on protein extractability. Thermal treatment for 30 min resulted in a significant reduction in SDS extractable glutenin level in wheat gluten. A significant drop in free sulphydryl level was found in wheat gluten treated at 70 °C for 30 min. It indicated that cross-linking of glutenin through S–S occurred during thermal treatment. The treatments at 70–90 °C led to significant decreases in soluble and nitrogen level, while significant increases in peptide nitrogen amount in the hydrolysates from treated gluten were found. A time-dependent effect was observed for the changes in soluble forms of nitrogen and PN. Thermal treatment resulted in molecular mass distribution change according to gel permeation chromatography analysis. Thermal treatment significantly increased the amount of fractions with molecular mass beyond 10 K (67.2%) in the hydrolysates and greatly decreased the amounts of fractions with MM of 10–5 K and below 5 K in hydrolysates. |
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Keywords: | Wheat gluten Enzymatic hydrolysis Thermal treatment Molecular mass distribution |
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