| 培养基和生长调节剂对油松离体胚不定芽诱导的影响 |
| |
| 引用本文： | 王永波,唐德瑞,马佩.培养基和生长调节剂对油松离体胚不定芽诱导的影响[J].北方园艺,2010(12):149-151. |
| |
| 作者姓名： | 王永波 唐德瑞 马佩 |
| |
| 作者单位： | 西北农林科技大学,林学院,陕西,杨凌,712100;西北农林科技大学,林学院,陕西,杨凌,712100;西北农林科技大学,林学院,陕西,杨凌,712100 |
| |
| 基金项目： | 国家林业局"948"资助项目 |
| |
| 摘 要： | 以油松成熟胚为外植体,对影响不定芽诱导分化的各因子做进一步试验。结果表明:油松成熟胚不定芽诱导的最佳组合为1/2MS+6-BA 1.5 mg/L+NAA 0.05 mg/L,诱导率为59.3%。对其进行趋势面分析,6-BA浓度为1.7 mg/L,NAA浓度为0.30~0.35 mg/L时不定芽诱导率可达65%。诱导阶段平均增殖率为8,愈伤组织增殖培养后平均增殖率最大可达13。
|
| 关 键 词： | 油松 成熟胚 组织培养 不定芽 |
The Influence of Medias and Growth Regulators on Explants to Induce Adventitious Buds of Pinus tabulaeformis |
| |
| WANG Yong-bo,TANG De-rui,MA Pei.The Influence of Medias and Growth Regulators on Explants to Induce Adventitious Buds of Pinus tabulaeformis[J].Northern Horticulture,2010(12):149-151. |
| |
| Authors: | WANG Yong-bo TANG De-rui MA Pei |
| |
| Institution: | WANG Yong-bo,TANG De-rui,MA Pei (College of Forestry,Northwest Agricultural and Forestry University,Yangling,Shaanxi 712100) |
| |
| Abstract: | The mature embryo of Pinus tabulaeformis was used as explants.This paper made a study of the facts of multiple adventitious shoot differentiation from callus cultures.The results showed that the combination of the medium was 1/2MS+6-BA 1.5 mg/L+NAA 0.2 mg/L,on which 59.3% of explants was induced.Applying the method of trend surface model,when 6-BA 1.7 mg/L and NAA 0.3~0.35.The highest induction rate of adventitious buds was 65%.Average rate of propagation was 8,after a generation of raise the highest averag... |
| |
| Keywords: | Pinus tabulaeformis mature embryo tissue culture adventitious buds |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
