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| 一株光合细菌分离鉴定及其降解甲氰菊酯特性 | |
| 引用本文： | 张战泓,罗源华,刘勇,张松柏,张德咏,罗香文.一株光合细菌分离鉴定及其降解甲氰菊酯特性[J].湖南农业大学学报(自然科学版),2009,35(4). |
| 作者姓名： | 张战泓 罗源华 刘勇 张松柏 张德咏 罗香文 |
| 作者单位： | 1. 湖南省农业科学院,蔬菜研究所,湖南,长沙410125 2. 湖南省农业科学院,植物保护研究所,湖南,长沙410125 3. 湖南省农业科学院,植物保护研究所,湖南,长沙410125;中南大学,研究生院隆平分院,湖南,长沙,410125 |
| 基金项目： | 国家"863"计划项目，国家科技支撑计划项目，湖南省农村科技计划重点项目 |
| 摘 要： | 从某农药厂的排放口污泥中筛选分离甲氰菊酯高效降解光合细菌PSB07-28,根据菌株生理生化特征以及16S rDNA序列同源性鉴定降解菌,气相色谱法测定降解甲氰菊酯的能力,渗透休克法提取降解粗酶,测定其降解能力,定位降解酶.结果表明,PSB07-28菌株属红假单胞菌属(Rhodopseudomonas sp),对甲氰菊酯的最高耐受质量浓度为800 mg/L,降解最佳条件为35 ℃,pH 7.在最佳降解条件下,光照培养15 d,对600 mg/L甲氰菊酯降解率达42.49%.降解酶定域结果表明,降解酶是胞内酶. |
| 关 键 词： | 甲氰菊酯 红假单胞菌属 生物学特性 生物降解 降解酶 |
Isolation,identification and degradation characteristics of fenpropathrin-degrading strain PSB07-28 |
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| ZHANG Zhan-hong,LUO Yuan-hua,LIU Yong,ZHANG Song-bai,ZHANG De-yong,LUO Xiang-wen.Isolation,identification and degradation characteristics of fenpropathrin-degrading strain PSB07-28[J].Journal of Hunan Agricultural University,2009,35(4). | |
| Authors: | ZHANG Zhan-hong LUO Yuan-hua LIU Yong ZHANG Song-bai ZHANG De-yong LUO Xiang-wen |
| Institution: | 1. a. Vegetable Institute;b. Plant Protection Institute;Hunan Academy of Agricultural Sciences;Changsha 410125;China;2.Branch of Longping;Graduate College;Central South University;China |
| Abstract: | A photosynthetic bacterial strain PSB07-28,capable of degrading fenpropathrin,was isolated by enrichment culture. PSB07-28 was identified based on its morphology,physiology and homologic analysis of 16S rDNA sequence(GenBank Accession No. EU004437) . With an evaluation of the degrading characteristics of this strain,an extraction of the degrading-enzyme crude and further testing of the degrading activity,the result shows PSB07-28 is identified as Rhodopseudomonas sp. The optimum conditions of degrading fenp... |
| Keywords: | fenpropathrin Rhodopseudomonas sp degradation characteristics biodegradation degrading-enzyme |
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