常绿阔叶杂交杜鹃组培体系的建立

常绿阔叶杂交杜鹃具有较高的观赏价值和园林利用价值，因而建立组培快繁体系具有重要意义。对8个材料的组培快繁研究结果表明，茎段外植体均能诱导萌发，平均诱导率达到75．2％，平均增殖系数达到5～6倍；以WPM为继代培养基的组培苗枝条粗壮、叶色深绿，优于Anderson、Read培养基；继代培养过程中的ZT浓度以0．5～1．0mg／L，pn值以4．8～5．2较为适宜；通过瓶外扦插生根方法获得了大量组培苗，其平均生根率达到76％左右，从而建立了常绿阔叶杂交杜鹃组培快繁体系。

In vitro proliferation of broadleaf evergreen rhododendron hybrids

It is of significance to establish in vitro culture system for broadleaf evergreen rhododendron hybrids since its high appreciation and landscape value. The shoot node as explants from eight hybrids in the experiment could be all induced in vitro culture, where the average induction rate was 75.2% while regeneration rate was a 5 - 6 fold. The shoots from WPM subculture medium showed the strongest growth and deepest green leaves compared to Anderson and Read medium. It was optimal at shoot proliferation stage that the hormone concentration of zeatin was at 0.5 - 1.0 mg/L and pH value was at 4.8 - 5.2. A large number of plantlets could be obtained by in vivo rooting with an average survival rate of 76%, hence in vitro rapid shoot proliferation culture was estabhshed.