| 利用cDNA-AFLP技术研究茶树花蕾发育基因差异表达片段 |
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| 引用本文： | 余梅,江昌俊,叶爱华,王朝霞,朱林,邓威威,高轩,宛晓春. 利用cDNA-AFLP技术研究茶树花蕾发育基因差异表达片段[J]. 茶叶科学, 2007, 27(3): 259-264. DOI: 10.13305/j.cnki.jts.2007.03.007 |
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| 作者姓名： | 余梅 江昌俊 叶爱华 王朝霞 朱林 邓威威 高轩 宛晓春 |
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| 作者单位： | 安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036;安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036;安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036;安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036;安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036;安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036;安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036;安徽农业大学茶叶生物化学和生物技术教育部、农业部重点实验室,安徽,合肥,230036 |
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| 摘 要： |
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| 关 键 词： | 花蕾发育 cDNA-AFLP 茶树 基因差异表达 |
| 文章编号： | 1000-369X（2007）03-259-06 |
| 收稿时间： | 2007-03-01 |
| 修稿时间： | 2007-03-012007-06-18 |
Analysis of Differential Gene Expression of Flower bud of Tea Plant (Camellia sinensis) by cDNA-AFLP |
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| YU Mei,JIANG Chang-jun,YE Ai-hua,WANG Zhao-xia,ZHU Lin,DENG Wei-wei,GAO Xuan,WAN Xiao-chun. Analysis of Differential Gene Expression of Flower bud of Tea Plant (Camellia sinensis) by cDNA-AFLP[J]. Journal of Tea Science, 2007, 27(3): 259-264. DOI: 10.13305/j.cnki.jts.2007.03.007 |
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| Authors: | YU Mei JIANG Chang-jun YE Ai-hua WANG Zhao-xia ZHU Lin DENG Wei-wei GAO Xuan WAN Xiao-chun |
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| Affiliation: | Key Laboratory of Tea Biochemistry & Biotechology of Ministry of Agriculture ﹠ Ministry of Education, Anhui Agricultural University, Hefei, 230036, China |
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| Abstract: | For studies of differential gene expression, cDNA-AFLP was analyzed in tea plant at the stage of bud flower development. The result showed that there were evident differential expression in flower between the early stage and the late stage. Of the 1110 fragments inspected, 35 and 87 specially displayed in the early and late stages; 15 fragments were cloned and sequenced. Sequence analysis indicated that among 15 squenced fragments, nine were sequence-similar to proteins in GenBank, i.e. oxidoreductase, chromosome 13, 14-3-3 protein, Amyrel gene, calreticulin interacted protein, ATP sulfurylase, catalytic / iron ion binding, desiccation-related protein and ntp302, two were similar to hypothetical proteins, and no similar sequence were found in GenBank for the other 4 fragment. |
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| Keywords: | bud flower development cDNA-AFLP tea plant(Camellia sinensis(L.)O. Kuntze cv. Wulong) gene differential expression |
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