| 盐酸四环素人工抗原的合成及鉴定 |
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| 引用本文： | 张桂贤. 盐酸四环素人工抗原的合成及鉴定[J]. 中国畜牧兽医, 2010, 37(3): 171-174 |
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| 作者姓名： | 张桂贤 |
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| 作者单位： | （山东临沂师范学院生命科学学院， 临沂 276005） |
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| 摘 要： | 本试验旨在合成盐酸四环素人工抗原,为其单克隆抗体的制备奠定试验基础。将盐酸四环素(TCH)发生霍夫曼降解和重氮化反应生成重氮盐,再与蛋白质载体(BSA,OVA)偶联生成免疫原和包被原,对偶联产物采用三氯化铁及紫外扫描法鉴定偶联成功,且免疫原和包被原的摩尔结合比分别约为3.4∶1和4.2∶1。用免疫原免疫BALB/c小鼠,用间接ELISA检测抗体效价,采用竞争ELISA法测IC50,获得了效价为1∶51200I、C50为16.91 ng/mL的多克隆抗体,最终证明人工抗原制备成功。
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| 关 键 词： | 盐酸四环素 霍夫曼 重氮化 偶联 载体 |
Synthesis and Identification of Artificial Antigen against Tetracycline Hydrochloride |
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| ZHANG Gui-xian. Synthesis and Identification of Artificial Antigen against Tetracycline Hydrochloride[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(3): 171-174 |
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| Authors: | ZHANG Gui-xian |
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| Affiliation: | （College of Life Science, Linyi Normal College, Linyi 276005,China） |
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| Abstract: | Hofmann degradation and diazo-reaction of Tetracycline hydrochloride (TCH) happened and conjugated to protein-carrier (BSA, OVA).The conjuagates were synthesized by the means of FeCl3 resolution and UV scanning spectra, and mol conjugation ration of TCH to BSA was 3.2∶1 and TCH to OVA was 4.2∶1.The immunogen(TCH-BSA) was immunized to BALB/c mice and polycolnal antibodies were obtained. Tiler of the antibodies were 1∶51200 by the indirect ELISA and IC50 was 16.91 ng/mL by the competitive indirect ELISA. It showed that the artificial-synthesized antigen had immunogenicity. |
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| Keywords: | Tetracycline hydrochloride Hofmann degradation diazo-reaction conjugation carrier |
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