黄瓜子房内源多胺含量的变化及其与单性结实的关系

以黄瓜(Cucumis sativus L.)单性结实品系EP-6与非单性结实品系ZR-2为试材,研究内源多胺含量与单性结实的关系。结果表明:内源多胺能够显著促进黄瓜子房的膨大以及初始阶段的发育。在果实发育过程中,EP-6未授粉处理的子房内源亚精胺(Spd)与精胺(Spm)水平显著高于授粉处理,而内源尸胺(Cad)与腐胺(Put)水平则显著低于授粉处理。对于ZR-2来说,Spd与Spm水平则是授粉处理高于未授粉处理,Cad与Put水平未授粉处理高于授粉处理。说明Spd与Spm有利于黄瓜果实的发育;Cad与Put则可能不利于果实发育。此外,黄瓜子房中多胺代谢趋势,尤其Spd与Spm的代谢与RNA和蛋白质代谢趋势基本一致,均在开花前后达到最高水平,然后逐渐下降,说明内源多胺可能主要是通过调节核酸与蛋白质的合成,从而促进黄瓜子房初始阶段的发育。

Relationship between endogeous polyamine contents in ovary and parthenocarpy in cucumber

In order to investigate the relationship between contents of endogeous polyamines in the ovary and parthenocarpy of cucumber,the parthenocarpic line EP-6 and non-parthenocarpic line ZR-2 were examined at different stages of ovary development.The results revealed that high levels of polyamines(PAs) could significantly promote cucumber ovary swelling and growth at early stage.In EP-6 line,spermidine(spd) and spermine(spm) levels in non-fertilized ovary were significantly higher those that in the fertilized one,and putrescine(put) and cadavarine(cad) levels in non-fertilized ovary were significantly lower than those in the fertilized one.However,in ZR-2 line,spd and spm levels were higher in fertilized ovary than those in non-fertilized one,and put and cad levels in fertilized one were lower than those in non-fertilized one.The results indicated that spd and spm were in favor of the development of cucumber ovary,and put and cad may be detrimental to the ovary development.Endogenous PAs(especially spd and spm) metabolism in cucumber ovary was the same as that of RNA and protein,reaching the highest level right before and after flowering,and then decreasing gradually.The results suggested that endogenous PAs especially spd and spm might stimulate cucumber ovary growth by regulating nucleic acid and protein synthesis.