| 黑曲霉有机磷农药降解酶的纯化及其性质 |
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| 引用本文： | 王继雯,甄静,谢宝恩,刘莹莹,李冠杰,周伏忠,陈国参.黑曲霉有机磷农药降解酶的纯化及其性质[J].江苏农业学报,2012,28(3):549-554. |
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| 作者姓名： | 王继雯 甄静 谢宝恩 刘莹莹 李冠杰 周伏忠 陈国参 |
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| 作者单位： | 1. 河南省微生物工程重点实验室,河南郑州450008;河南省科学院生物研究所有限责任公司,河南郑州450008
2. 河南省微生物工程重点实验室,河南郑州,450008 |
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| 基金项目： | 2009年河南省重大公益性科研招标项目 |
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| 摘 要： | 采用细胞破碎、硫酸铵分级沉淀和Sephadex G-75凝胶过滤的方法,对黑曲霉(Aspergillus niger)J6产生的有机磷农药降解酶进行分离和纯化,并对其酶学性质进行研究。结果显示:黑曲霉J6有机磷农药降解酶定位在细胞膜上,其分子量为66 000。该降解酶最适pH值为7.0,最适反应温度为70℃,Fe3+、Cu2+、Li+、Zn2+、Mg2+、NaN3、EDTA、SDS、PSMF对该降解酶有不同程度的激活作用,而Mn2+对其有轻微的抑制作用,Ca2+和Triton对其有强烈的抑制作用。
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| 关 键 词： | 黑曲霉 有机磷农药降解酶 酶学性质 |
Purification of a organophosphorus pesticide-degrading enzyme from Aspergillus niger J6 and its properties |
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| WANG Ji-wen
,
ZHEN Jing
,
XIE Bao-en
,
LIU Ying-ying
,
LI Guan-jie
,
ZHOU Fu-zhong
,
CHEN Guo-can.Purification of a organophosphorus pesticide-degrading enzyme from Aspergillus niger J6 and its properties[J].Jiangsu Journal of Agricultural Sciences,2012,28(3):549-554. |
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| Authors: | WANG Ji-wen ZHEN Jing XIE Bao-en LIU Ying-ying LI Guan-jie ZHOU Fu-zhong CHEN Guo-can |
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| Institution: | 1(1.Key Laboratory of Microbial Engineering of Henan Province,Zhengzhou 450008,China;2.Biological Research Institute Co.Ltd.,Henan Academy of Sciences,Zhengzhou 450008,China) |
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| Abstract: | A organophosphorus hydrolase from strain Aspergillus niger J6 was isolated and purified by means of cell disruption,ammonium sulfate precipitation and Sephadex G-75 chromatography,and its enzymatic properties were studied.The results showed that the degradation enzyme was located on the cell membrane,with its molecular weight being 66 000 by SDS-PAGE.The optimal reactive temperature and pH of the degradation enzyme were 70 ℃ and pH 7.0.Some metal ions(Fe3+,Cu2+,Li+,Zn2+,Mg2+) and chemical reagents(NaN3,EDTA,SDS and PSMF) showed activation on the degradation enzyme,while Mn2+ displayed slight inhibition,and Ca2+,Triton had strong inhibition. |
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| Keywords: | Aspergillus niger organophosphorus pesticide-degrading enzyme enzymatic property |
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