不同培养方法对"三峡一号"猕猴桃无菌苗生长影响的研究

试验结果表明 ,在“三峡一号”猕猴桃组织培养中半固体培养不降低无菌苗生长势 ,对无菌苗生长有一定促进作用 ,适合于“三峡一号”无菌苗的快速繁殖。半固体培养 2 8d比相同条件下固体培养的无菌苗分化植株高出 1 2 0 % ,平均植株高度高出 68 84% ,植株叶片数高出1 1 5 1 5 % ,单叶面积高出 36 1 2 % ,经方差分析 ,前三者差异极显著 ,单叶面积差异显著 ,具有统计学意义 ;半固体培养 60d比相同条件下固体培养的无菌苗植株高度、单株叶片数、单叶面积分别高出 81 6%、5 7 4%、35 98% ,经方差分析 ,前三者差异极显著 ,具有统计学意义。

Effect of Different Culture Methods on Sterile Seedling's Growth of "Sanxia No. 1" Yangtao Actinidia

The test results showed that semisolid culture is one of tissue culture method of “Sanxia No.1” Yangtao Actinidia which don't decrease growth potential, have some promoting action on growth and is suitable for fast reproduction for this kind of sterile seedling. Compared with solid culture under the same condition for 28 days or 60 days (28/60). The semisolid culture's sterile seedling are 68 84%/81 6%, 115 15%/57 4%, 36 12%/35 98% higher in plant height, leaf number per plant, leaf area per leaf respectively. The plant number differentiated from 28 days of semisolid culture is 120% higher than from 28 days of solid culture. All results have statistical means because the differences between solid culture and semisolid culture are extremely significant (plant height and leaf number per plant (28/60), plant number differentiated (28 days) and leaf area per leaf (60 days)) or is significant (leaf area per leaf (28 days) in the stand-point of variance analysis.