| 光皮树优良无性系组织培养的无菌体系建立 |
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| 引用本文： | 本玛丽,王晓明,李永欣,曾慧杰,蔡能. 光皮树优良无性系组织培养的无菌体系建立[J]. 湖南林业科技, 2010, 37(2): 5-7,11 |
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| 作者姓名： | 本玛丽 王晓明 李永欣 曾慧杰 蔡能 |
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| 作者单位： | 中南林业科技大学,湖南,长沙,410004;中南林业科技大学,湖南,长沙,410004;湖南省林业科学研究院,湖南,长沙,410004;林木无性系育种湖南省重点实验室,湖南,长沙,410004;湖南省林业科学研究院,湖南,长沙,410004;林木无性系育种湖南省重点实验室,湖南,长沙,410004 |
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| 摘 要： | 以光皮树优良无性系的嫩枝茎段为试验材料,研究不同时期的外植体、灭菌处理与植物激素对接种污染率和无菌外植体得率的影响。结果表明,秋季带顶芽的嫩茎为接种培养的适宜外植体,外植体最佳消毒方法为75%酒精浸泡10 s后,用2~3滴吐温80的0.1%升汞溶液消毒7~9 min。试验获得了光皮树适宜的初代培养基:MS+6-BA 2.0 mg.L-1+NAA 0.05 mg.L-1+PVP 300 mg.L-1+蔗糖30 g.L-1。
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| 关 键 词： | 光皮树 组织培养 污染率 外植体 |
Germfree system establishment of tissue culture from the superior clone of Cornus wilsoniana |
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| BEN Mali,WANG Xiaoming,LI Yongxin,ZENG Huijie,CAI Neng. Germfree system establishment of tissue culture from the superior clone of Cornus wilsoniana[J]. Hunan Forestry Science & Technology, 2010, 37(2): 5-7,11 |
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| Authors: | BEN Mali WANG Xiaoming LI Yongxin ZENG Huijie CAI Neng |
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| Affiliation: | BEN Mali1,WANG Xiaoming1,2,3,LI Yongxin2,ZENG Huijie2,CAI Neng2,3(1.Central South University of Forestry and Technology,Changsha 410004,China,2.Hunan Forestry Academy,3.Hunan Key Laboratory of Trees Clones Breeding Technology,China) |
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| Abstract: | Taken young stem segments of the superior clone of Cornus wilsoniana as experimental materials,the effects of different explants,sterilization treatment and phytohormone on pollution rate and germfree explants rate were researched.The results showed that the suitable explants were the segments of young stem with terminal bud in autumn.And the optimum explants sterilization method was to dip in 75% alcohol for 10 s,then in 0.1% HgCl2 with 2~3 drops tween-80 for 7~9 min.The suitable medium for primary culture... |
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| Keywords: | Cornus wilsoniana tissue culture pollution rate explants |
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