| 检测犬源狂犬病毒中和抗体ELISA方法的建立 |
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| 引用本文： | 傅秋玲,郑佳琳,林颖仪,张 莹,阳佑天,潘姣姣,吴晓薇,郭霄峰.检测犬源狂犬病毒中和抗体ELISA方法的建立[J].华南农业大学学报,2012,33(4):561-565. |
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| 作者姓名： | 傅秋玲 郑佳琳 林颖仪 张 莹 阳佑天 潘姣姣 吴晓薇 郭霄峰 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 基金项目： | 广东省省部产学研重点项目，农业公益性行业专项 |
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| 摘 要： | 为了有效监测犬免疫狂犬病疫苗后的保护效力,以狂犬病毒(Rabies virus,RV)糖蛋白的主要优势抗原表位区G3蛋白(RV G3)作为包被抗原,建立了一种检测狂犬病毒中和抗体效价的间接ELISA方法.通过优化反应条件,确定抗原最佳包被量为8 mg/L,血清的最佳稀释度为1∶100,酶标二抗的稀释度为1∶3 000.特异性试验表明,该抗原不与犬瘟热病毒、犬腺病毒、犬细小病毒阳性血清发生交叉反应;批内和批间重复性试验的平均变异系数都小于10%;敏感性达1∶1 280.此方法检测134份血清样品的结果与美国SYNBIOTICS试剂盒相比,总符合率达95.6%.
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| 关 键 词： | 狂犬病毒 糖蛋白 犬源 中和抗体 间接ELISA |
| 收稿时间： | 2012/4/10 0:00:00 |
Development of an ELISA for Detection of Neutralizing Antibodies Against Canine Rabies Virus |
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| FU Qiu-ling,ZHENG Jia-lin,LIN Ying-yi,ZHANG Ying,YANG You-tian,PAN Jiao-jiao,WU Xiao-wei and GUO Xiao-feng.Development of an ELISA for Detection of Neutralizing Antibodies Against Canine Rabies Virus[J].Journal of South China Agricultural University,2012,33(4):561-565. |
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| Authors: | FU Qiu-ling ZHENG Jia-lin LIN Ying-yi ZHANG Ying YANG You-tian PAN Jiao-jiao WU Xiao-wei and GUO Xiao-feng |
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| Institution: | College of Veterinary Medicine, South China Agricultural University,;College of Veterinary Medicine, South China Agricultural University,;College of Veterinary Medicine, South China Agricultural University,;College of Veterinary Medicine, South China Agricultural University,;College of Veterinary Medicine, South China Agricultural University,;College of Veterinary Medicine, South China Agricultural University,;College of Veterinary Medicine, South China Agricultural University,;College of Veterinary Medicine, South China Agricultural University, |
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| Abstract: | |
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| Keywords: | rabies virus glycoprotein canine neutralizing antibodies indirect ELISA |
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