| 不同猪苓菌核分离营养菌丝的研究 |
| |
| 引用本文： | 陈永刚,邓百万,陈文强,彭浩,刘开辉,魏巍,刘飞.不同猪苓菌核分离营养菌丝的研究[J].安徽农业科学,2007,35(28):8840-8841. |
| |
| 作者姓名： | 陈永刚 邓百万 陈文强 彭浩 刘开辉 魏巍 刘飞 |
| |
| 作者单位： | 陕西东盛生物制品有限公司,陕西汉中,723000;陕西理工学院生物科学与工程学院,陕西汉中,723001 |
| |
| 基金项目： | 陕西教育厅重点实验室重点科研项目（04JS32）. |
| |
| 摘 要： | 目的]为了明确猪苓的种质资源,为其大面积栽培提供理论依据。方法]采用组织培养法,分离秦巴山区3种猪苓营养菌丝。结果]结果表明,在温度25~28℃、pH值自然的条件下,用CPDA培养基和蛋白胨培养基培养,不同猪苓菌核均能分离出营养菌丝。结论]综合不同猪苓菌核分离营养菌丝的萌发时间和萌发率、菌丝密度和生长势等因素,发现CPDA培养基较适合作为分离培养基。
|
| 关 键 词： | 猪苓 菌核 分离 营养菌丝 |
| 文章编号： | 0517-6611（2007）28-08840-02 |
| 修稿时间： | 2007-07-17 |
Research on Separating Vegetative Hyphae from Different Polyporus umbellate Sclerotia |
| |
| CHEN Yong-gang et al.Research on Separating Vegetative Hyphae from Different Polyporus umbellate Sclerotia[J].Journal of Anhui Agricultural Sciences,2007,35(28):8840-8841. |
| |
| Authors: | CHEN Yong-gang |
| |
| Institution: | Shaanxi Topsun Biological Products Co.Ltd.;Hanzhong;Shaanxi 723000 |
| |
| Abstract: | Objective] The aim was to confirm the germplasm resources so as to provide theoretical foundation for its cultivation in large area.Method] The vegetative hypha of 3 different varieties of Polyporus umbellate from Qinba mountainous areas were separated by tissue culture method.Result] Under the temperature of 25～28 ℃ and the natural pH condition,the vegetative hypha could be separated from different P.umbellate sclerotia by using CPDA medium and peptone medium.Conclusion] Integrating the factors such as germination time,germination rate,hyphae density and growth of the vegetative hyphae separated from different P.umbellate sclerotia,CPDA medium was more suitable to be as the separating medium. |
| |
| Keywords: | Polyporus umbellate Sclerotia Separation Vegetative hyphae |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
