| 柱花草花药离体培养研究 |
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| 引用本文： | 单国燕,;郑金龙,;高建明,;陈河龙,;张世清,;易克贤.柱花草花药离体培养研究[J].热带农业科学,2014(4):36-41. |
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| 作者姓名： | 单国燕 ;郑金龙 ;高建明 ;陈河龙 ;张世清 ;易克贤 |
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| 作者单位： | [1]中国热带农业科学院环境与植物保护研究所,海南海口571101; [2]农业部热带作物生物技术重点开放实验室,海南海口571101; [3]中国热带农业科学院热带生物技术研究所,海南海口571101 |
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| 基金项目： | 国家自然科学基金“柱花草炭疽菌致病相关基因的克隆及功能分析”（No.31072076）：公益性行业科研专项“草地病害防治技术研究与示范”（No.201303057）;国家科技支撑计划“热带亚热带地区超高产牧草新品种选育及产业化示范”（No.2008BADB3808）;科技部国家科技成果重点推广计划项目“高蛋白热带豆科牧草配套养殖奶牛产业示范”（No.05EFN216900381）. |
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| 摘 要： | 以热研2号柱花草花药为外植体,研究不同植物生长调节剂、培养基以及低温预处理等对柱花草花药培养的影响,以期建立柱花草花药培养体系,获得再生植株,为柱花草倍性、基因组学及分子生物学研究提供材料。结果表明:花药4℃低温预处理24 h诱导效果较好;N6培养基对愈伤组织的诱导效果比MS培养基好;最佳愈伤组织诱导培养基组合为N6+2,4-D 0.5 mg/L+KT1.0 mg/L;分化培养的最佳组合为MS+NAA 0.2 mg/L+6-BA 3.0 mg/L。
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| 关 键 词： | 柱花草 花药培养 愈伤组织诱导 不定芽分化 |
In Vitro Anther Culture of Stylosanthes |
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| Institution: | SHAN Guoyan, ZHENG Jinlong, GAO Jianming, CHEN Helong, ZHANG Shiqing, YI Kexian (1 Environment and Plant Protection Institute, CATASs, Haikou, Hainan 571101, P.R. China; 2 Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology, Haikou, Hainan 571101; Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101, P.R. China) |
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| Abstract: | In order to establish an efficient anther culture system of Stylosanthes, get regenerate plantlet and provide basic materials for the research of ploidy, genomics and molecular biology of Stylosanthes. This experiment taking the anther of Stylosanthes guianensis cv, Reyan2 as explants to study the effect of plant growth regulator, medium and low temperature pretreatment on Stylosanthes anther culture. The results indicated that low temperature pretreatment for 24 h was better than other treatments. N6 medium supplemented with 2,4-D 0.5 mg/L, KT 1.0 mg/L was the best for induction of cullus. MS＋NAA 0.2 mg/L＋6-BA 3.0 mg/L was the best for regeneration culture. |
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| Keywords: | Stylosanthes anther culture callus induction division of adventitious bud |
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