| 高能饲粮对育成期蛋鸡肝脏miRNA表达谱的影响 |
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| 引用本文： | 王星果,卢建,关树勇,曲亮,孙平江,郭军,胡玉萍,窦套存,马猛,李永峰,王克华. 高能饲粮对育成期蛋鸡肝脏miRNA表达谱的影响[J]. 动物营养学报, 2021, 33(2): 1081-1090 |
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| 作者姓名： | 王星果 卢建 关树勇 曲亮 孙平江 郭军 胡玉萍 窦套存 马猛 李永峰 王克华 |
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| 作者单位： | 江苏省家禽科学研究所,扬州 225125;江苏省家禽科学研究所,扬州 225125;南京农业大学,南京 210095;江苏峪口禽业有限公司,宿迁 223733 |
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| 基金项目： | 江苏省家禽遗传育种重点实验室资助项目;省属公益类科研院所自主科研经费;现代农业产业技术体系建设专项;江苏省农业重大新品种创制项目;江苏省自然科学基金青年项目 |
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| 摘 要： | 本研究利用高能和低能饲粮饲喂育成期蛋鸡,运用small RNA测序方法对肝脏中miR-NA进行检测,对其表达量进行分析,对差异表达miRNA进行靶基因预测,并对差异表达的预测靶基因进行基因本体论(GO)功能和京都基因与基因组百科全书(KEGG)信号通路富集分析.结果表明:与低能组相比,高能组有2个miRNA表达显著上调...
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| 关 键 词： | 育成期 高能饲粮 蛋鸡 肝脏 miRNA |
Effects of High Energy Diet on Liver miRNA Expression Profile of Laying Hens during Growing Period |
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| WANG Xingguo,LU Jian,GUAN Shuyong,QU Liang,SUN Pingjiang,GUO Jun,HU Yuping,DOU Taocun,MA Meng,LI Yongfeng,WANG Kehua. Effects of High Energy Diet on Liver miRNA Expression Profile of Laying Hens during Growing Period[J]. CHINESE JOURNAL OF ANIMAL NUTRITION, 2021, 33(2): 1081-1090 |
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| Authors: | WANG Xingguo LU Jian GUAN Shuyong QU Liang SUN Pingjiang GUO Jun HU Yuping DOU Taocun MA Meng LI Yongfeng WANG Kehua |
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| Affiliation: | (Jiangsu Institute of Poultry Science,Yangzhou 225125,China;Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Yukou Poultry Industry Co.,Ltd.,Suqian 223733,China) |
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| Abstract: | In current study,miRNAs in liver were detected by small RNA sequencing of laying hens fed with high-energy or low-energy during the growing period,and their expression levels were analyzed.Target genes of differentially expressed miRNAs were predicted,and differentially expressed target genes were used to undergo gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis.The results showed that compared with low energy group,the expression levels of 2 miRNAs in high energy group were up-regulated and 6 miRNAs were down-regulated(P<0.05).Forty target genes of differentially expressed miRNAs were predicted in differentially expressed genes,and the number of predicted target genes of miR-15a was the biggest for 26.Analysis of GO and KEGG of differentially expressed target genes showed that immune and regulation of molecular function were the most enriched processes,and the significantly enriched signaling pathways were related with immune and lipid metabolism.It is indicates that energy intake regulates immune and lipid metabolism of laying hens by influencing expression of miRNAs and then upregulates or downregulates expression of immune and lipid metabolism related target genes in liver,and finally affects production performance. |
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| Keywords: | growing period high energy diet laying hen liver miRNAs |
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