| The Effects of Dwarfing Genes （Rht-B1b,Rht-D1b,and Rht8） with Different Sensitivity to GA3 on the Coleoptile Length and Plant Height of Wheat |
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| 引用本文： | TANG Na,JIANG Ying,HE Bei-ru,HU Yin-gang. The Effects of Dwarfing Genes （Rht-B1b,Rht-D1b,and Rht8） with Different Sensitivity to GA3 on the Coleoptile Length and Plant Height of Wheat[J]. 中国农业科学(英文版), 2009, 8(9): 1028-1038. DOI: 10.1016/S1671-2927(08)60310-7 |
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| 作者姓名： | TANG Na JIANG Ying HE Bei-ru HU Yin-gang |
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| 作者单位： | TANG Na,JIANG Ying,HE Bei-ru(College of Agronomy,Northwest A&F University,Yangling 712100,P.R.China);HU Yin-gang(College of Agronomy,Northwest A&F University,Yangling 712100,P.R.China;Yangling Branch of China National Wheat Improvement Centre,Yangling 712100,P.R.China;Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling 712100,P.R.China) |
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| 基金项目： | This research was supported by the National High-Tech R&D Program of China，the National Basic Research Program of China，the 111 Program of Introducing Talents of Discipline to Universities of China，the ACIAR Program of Australia |
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| 摘 要： | Understanding the effects of wheat dwarfing genes on the coleoptile length and plant height is crucial for the proper utilization of dwarfing genes in the improvement of wheat yield. Molecular marker analysis combined with pedigree information were used to classify wheat cultivars widely planted in major wheat growing regions in China into different categories based on the dwarfing genes they carried. The effects of the dwarfing genes with different sensitivity to gibberellins （GA3） on the coleoptile length and plant height were analyzed. Screening of 129 cultivars by molecular marker analysis revealed that 58 genotypes of wheat contained the dwarfing gene Rht-B1b, 24 genotypes of wheat contained Rht-D1b gene and 73 genotypes of wheat possessed Rht8 gene. In addition, among these 129 cultivars, 35 genotypes of wheat cultivars contained both Rht-B1b and Rht8 genes and 16 genotypes of wheat cultivars contained both Rht-D1b and Rht8 genes. Wheat cultivars with the dwarfing genes Rht-B1b or Rht-D1b were insensitive to GA3, while the cultivars with the dwarfing gene Rht8 were sensitive to GA3. Most of the wheat genotypes containing combination of Rht8 gene with either Rht-B1b or Rht-D1b gene were insensitive to GA3. The plant height was reduced by 24.6, 30.4, 28.2, and 32.2%, respectively, for the wheat cultivars containing Rht-B1b, Rht-D1b, Rht-B1b ＋ Rht8, and Rht-D1b ＋ Rht8 genes. The plant height was reduced by 14.3% for the wheat cultivar containing GA3-sensitive gene Rht8. The coleoptile length was shortened by 25.4, 31.3, 28.4 and 31.3%, respectively, in the wheat cultivars containing Rht-B1b, Rht-D1b, Rht-B1b ＋Rht8 and Rht-D1b ＋ Rht8 genes, while the coleoptile length was shortened only by 6.2% for the wheat cultivar containing Rht8 gene. We conclude that GA3-insensitive dwarfing genes （Rht-B1b and Rht-D1b） are not suitable for the wheat improvement in dryland because these two genes have effect on reducing both plant height and coleoptile length. In contrast, GA3- sensitive dwarfing gene （Rht8） is a relatively ideal candidate for the wheat improvement since it significantly reduces the plant height of wheat, but has less effect on the coleoptile length.
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| 关 键 词： | 胚芽鞘 小麦 生产 产量 |
The Effects of Dwarfing Genes (Rht-B1b,Rht-D1b,and Rht8) with Different Sensitivity to GA3 on the Coleoptile Length and Plant Height of Wheat |
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| TANG Na,JIANG Ying,HE Bei-ru,HU Yin-gang. The Effects of Dwarfing Genes (Rht-B1b,Rht-D1b,and Rht8) with Different Sensitivity to GA3 on the Coleoptile Length and Plant Height of Wheat[J]. 《Agricultural Sciences in China》, 2009, 8(9): 1028-1038. DOI: 10.1016/S1671-2927(08)60310-7 |
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| Authors: | TANG Na JIANG Ying HE Bei-ru HU Yin-gang |
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| Affiliation: | 1. College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China;2. Yangling Branch of China National Wheat Improvement Centre, Yangling 712100, P.R.China;3. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, P.R.China;1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China;2. College of Resources, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China;1. Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China;1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;2. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271018, Shandong, China;3. Novogene Bioinformatics Technology Co., Ltd., Beijing 100015, China;1. Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;2. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), D-06466 Stadt Seeland, OT Gatersleben, Germany;3. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria;1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;2. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;3. Novogene Bioinformatics Institute, Beijing 100083, China;4. Collaborative Innovation Center of Crop Stress Biology & Institute of Plant Stress Biology, School of Life Science, Henan University, Kaifeng 475004, China;5. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China |
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| Abstract: | Understanding the effects of wheat dwarfing genes on the coleoptile length and plant height is crucial for the proper utilization of dwarfing genes in the improvement of wheat yield.Molecular marker analysis combined with pedigree information were used to classify wheat cultivars widely planted in major wheat growing regions in China into different categories based on the dwarfing genes they carried.The effects of the dwarfing genes with different sensitivity to gibberellins (GA3) on the coleoptile length and plant height were analyzed.Screening of 129 cultivars by molecular marker analysis revealed that 58 genotypes of wheat contained the dwarfing gene Rht-B1b,24 genotypes of wheat contained Rht-D1b gene and 73 genotypes of wheat possessed Rht8 gene.In addition,among these 129 cultivars,35 genotypes of wheat cultivars contained both Rht-B1b and Rht8 genes and 16 genotypes of wheat cultivars contained both Rht-D1b and Rht8 genes.Wheat cultivars with the dwarfing genes Rht-B1b or Rht-D1b were insensitive to GA3,while the cultivars with the dwarfing gene Rht8 were sensitive to GA3.Most of the wheat genotypes containing combination of Rht8 gene with either Rht-B1b or Rht-Dlb gene were insensitive to GA3.The plant height was reduced by 24.6,30.4,28.2,and 32.2%,respectively,for the wheat cultivars containing Rht-B1b,Rht-D1b,Rht-B1b+Rht8,and Rht-D1b+Rht8 genes.The plant height was reduced by 14.3% for the wheat cultivar containing GA3-sensitive gene Rht8.The coleoptile length was shortened by 25.4,31.3,28.4 and 31.3%,respectively,in the wheat cultivars containing Rht-B1b,Rht-D1b,Rht-B1b+Rht8 and Rht-D1b+Rht8 genes,while the coleoptile length was shortened only by 6.2% for the wheat cultivar containing Rht8 gene.We conclude that GA3-insensitive dwarfing genes (Rht-B1b and Rht-D1b)are not suitable for the wheat improvement in dryland because these two genes have eflfect on reducing both plant height and coleoptile length.In contrast,GA3-sensitive dwarfing gene (Rht8) is a relatively ideal candidate for the wheat improvement since it significantly reduces the plant height of wheat,but has less effect on the coleoptile length. |
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| Keywords: | bread wheat dwarfing genes Rht-B1b Rht-D1b Rht8 coleoptile length plant height |
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