| 根癌农杆菌介导的苦瓜遗传转化 |
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| 引用本文： | 林义章,罗燕华. 根癌农杆菌介导的苦瓜遗传转化[J]. 福建农林大学学报(自然科学版), 2007, 36(2): 147-150 |
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| 作者姓名： | 林义章 罗燕华 |
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| 作者单位： | 福建农林大学园艺学院,福建,福州,350002 |
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| 摘 要： | 用含质粒载体pB I121的根癌农杆菌转化“南屿”苦瓜子叶节,通过对gus基因瞬时表达情况进行观测,对根癌农杆菌介导的苦瓜遗传转化体系相关因素进行研究.结果表明,苦瓜子叶节必须预培养3 d左右,用D600nm值为0.3-0.5之间的菌液浸泡,侵染时间为10 min,然后共培养3-4 d.再生植株经PCR检测初步证明gus基因已成功整合到苦瓜基因组中.
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| 关 键 词： | 苦瓜 子叶节 根癌农杆菌 gus瞬时表达 |
| 文章编号： | 1671-5470(2007)02-0147-04 |
| 修稿时间： | 2006-07-07 |
Agrobacterium tumefaciens mediated genetic transformation of Momordica charantia L. |
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| LIN Yi-zhang,LUO Yan-hua. Agrobacterium tumefaciens mediated genetic transformation of Momordica charantia L.[J]. Journal of Fujian Agricultural and Forestry University, 2007, 36(2): 147-150 |
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| Authors: | LIN Yi-zhang LUO Yan-hua |
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| Affiliation: | College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
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| Abstract: | The cotyledon nodes of Nanyu balsam pear were used as the receptors for transformation by Agrobacterium tumefaciens containing pBI121.By detecting the transient expression of gus gene,this experiment investigated the correlative factors affecting Agrobacterium-mediated transformation of balsam pear.The optimum conditions for the transformation were as follows: preculture duration about 3 days,bacterium density of D600 nm 0.3-0.5,infection duration 10 min,and coculture duration 3-4 days.The presence of gus gene was detected by PCR. |
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| Keywords: | Momordica charantia L. cotyledon node Agrobacterium tumefaciens gus transient expression |
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