| 鹅细小病毒VP1-VP3非重叠区基因的克隆与序列分析 |
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| 引用本文： | 王志强,刘力威,杨旭东,李洪彬,黄芳芳,邹跃,陈亮,黄宇翔.鹅细小病毒VP1-VP3非重叠区基因的克隆与序列分析[J].中国家禽,2011,33(19). |
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| 作者姓名： | 王志强 刘力威 杨旭东 李洪彬 黄芳芳 邹跃 陈亮 黄宇翔 |
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| 作者单位： | 1. 黑龙江省兽医科学研究所,黑龙江齐齐哈尔,161006
2. 东北农业大学动物科学技术学院,黑龙江哈尔滨,150030 |
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| 摘 要： | 通过克隆鹅细小病毒(Goose parvovirus,GPV)分离株VP1-V3非重叠区基因,并对其进行序列分析,为鹅细小病毒感染与疫苗免疫的鉴别诊断奠定理论基础.进行鹅胚病毒增殖,收集尿囊液,提取基因组,参考发表的B株序列,设计合成一对引物,经PCR扩增,克隆VP1-VP3基因,筛选阳性克隆,对其进行序列测定及同源性分析.结果表明,克隆的基因片段为901 bp,VP1-VP3基因共594 bp,编码198个氨基酸；弱毒株之间亲缘关系很近,核苷酸同源性99.5％～100％,氨基酸同源性98.5％～100％；强毒株与B株亲缘关系较近,核苷酸同源性96.6％,氨基酸同源性97.5％；弱毒株与强毒株亲缘关系较远,核苷酸同源性92％～93％,氨基酸同源性96％～97.5％；弱毒株与B株亲缘关系最远,核苷酸同源性92.5％～93％,氨基酸同源性93.9％～95.5％.说明强毒株与弱毒株之间核苷酸序列存在差异.
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| 关 键 词： | 鹅细小病毒 VP1 -VP3非重叠区基因 克隆 序列分析 |
Cloning and Sequencing of Non-repeated VP1 and VP3 Gene of Goose Parvovirus |
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| WANG Zhiqiang,LIU Liwei,YANG Xudong,LI Hongbin,HUANG Fangfang,ZOU Yue,CHEN Liang,HUANG Yuxiang.Cloning and Sequencing of Non-repeated VP1 and VP3 Gene of Goose Parvovirus[J].China Poultry,2011,33(19). |
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| Authors: | WANG Zhiqiang LIU Liwei YANG Xudong LI Hongbin HUANG Fangfang ZOU Yue CHEN Liang HUANG Yuxiang |
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| Institution: | WANG Zhiqiang~1,LIU Liwei~1,YANG Xudong~1,LI Hongbin~1,HUANG Fangfang~2,ZOU Yue~1,CHEN Liang~1,HUANG Yuxiang~1(1.Veterinary Science Research Institute of Heilongjiang Province,Qiqihaer,Heilongjiang 161006,2.College of Animal Science and Technology,Northeast Agricultural University,Harbin,Heilongjiang 150030) |
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| Abstract: | A pair of specific primers was designed according to the gene sequence of goose parvovirus(GPV) B strain and used to amplify VP1-VP3 gene by PCR,sequence and analyze for homology.The result of sequencing showed that the product of PCR of VP1-VP3 gene from GPV B strain was consisted of 594 bases,encode 198 amino acids.The homology of nucleotides of four isolated low virulent strains was 99.5% to 100%;the homologies of nucleotides of standard B strains were 96.6% to that of isolated virulent strain,92.5% to 9... |
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| Keywords: | Goose parvovirus(GPV) non-repeated VP1 and VP3 gene cloning sequencing |
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