不同地理群体乌鳢线粒体DNA控制区结构分析及遗传多样性

为研究乌鳢群体的遗传多样性和控制区结构,实验采用PCR和DNA测序技术对其线粒体DNA控制区序列进行比较。结果显示,用于分析的线粒体DNA控制区全长序列为905~908 bp。104个序列中共发现了37个多态位点,定义了27种单倍型。同时对控制区结构进行分析,识别了其终止序列区(ETAS)、中央保守区(CD)和保守序列区(CSB)的关键序列。3个地理群体的单倍型多样性(Hd)、核苷酸多样性(Pi)和平均核苷酸差异数(k)分别为0.875、0.003 27和2.939。群体间的平均Kimura双参数遗传距离(Kimura 2-parameter distance,K 2-P)、遗传分化指数(Fst)、基因交流值(Nm)和分子方差分析(AMOVA)均表明,3个乌鳢群体具有较高的遗传分化,白洋淀群体和平原县群体间存在一定的基因交流。

Structure analysis of mtDNA D-Loop region and the genetic diversity of Channa argu in different populations

PCR and DNA sequencing technology were used to compare the DNA sequence of mitochondrial control region of 3 Channa argu populations to study the structure and the genetic diversity.The result shows that the sequence length of the D-Loop region which was used for analysis is 905-908 bp.37 polymorphic sites were found in 104 sequences and 27 haplotypes were defined.Meanwhile,the control region was analyzed,including terminal associated sequence,central conserved domains and conserved sequence block domains.The haploid type diversity(H_d),nucleotide diversity(P_i)and the average number of nucleotide differences(k)of 3 geographical populations were 0.875,0.003 27 and 2.939 respectively.Average Kimura double parameters of genetic distance between populations,genetic differentiation index(F_st),gene exchange value(N_m)and Analysis of Molecular Variance(AMOVA)all indicated that 3 populations of C.argu have high genetic differentiation.The Baiyangdian population and Pinyuan County population had certain gene exchange.