| 干酪乳杆菌LC-03的培养条件优化研究 |
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| 引用本文： | 金桩,彭健,胡新文,林声汉,马燕. 干酪乳杆菌LC-03的培养条件优化研究[J]. 中国畜牧兽医, 2010, 37(6): 223-226 |
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| 作者姓名： | 金桩 彭健 胡新文 林声汉 马燕 |
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| 作者单位： | (1.海南省海口市罗牛山股份有限公司博士后科研工作站， 海口 570125； 2.华中农业大学， 武汉 430070；3.海南大学， 海口 570228； 4.海南省畜牧兽医技术推广站， 海口 570168） |
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| 基金项目： | 海南省重点科技计划项目 |
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| 摘 要： | 本研究通过单因子优化试验和正交试验对干酪乳杆菌LC-03培养条件和培养基成分进行初步优化。结果表明,干酪乳杆菌LC-03属于兼性厌氧菌,最佳培养基成分为:蛋白胨12g,酵母提取物6g,牛肉膏6g,葡萄糖18g,乙酸钠5g,柠檬酸铵2.15g,Tween801mL,MgSO4.7H2O0.58g,MnSO4.4H2O0.05g,K2HPO42g,水1000mL;最佳培养条件:温度为35℃,培养基起始pH为6.5,接种量为1.0%,培养时间为24h;优化培养后D610nm值达到1.832,活菌数达到3.22×1010CFU/mL。
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| 关 键 词： | 干酪乳杆菌LC-03 培养条件 培养基成分 优化 D610 nm值 活菌数 |
The Optimization of Culture Conditions of the Lactobacillus caseis-03 |
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| JIN Zhuang,PENG Jian,HU Xin-wen,LIN Sheng-han,MA Yan. The Optimization of Culture Conditions of the Lactobacillus caseis-03[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(6): 223-226 |
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| Authors: | JIN Zhuang PENG Jian HU Xin-wen LIN Sheng-han MA Yan |
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| Affiliation: | (1.Haikou Agriculture & Industry & Trade Luoniushan Co.Ltd. Postdoctoral Programmer, Haikou 570125, China; 2.Huazhong Agricultural University, Wuhan 430070, China; 3.Hainan University, Haikou 570228, China; 4.Hainan Animal and Veterinarian Science Technology Extention Station, Haikou 570168, China) |
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| Abstract: | In this study the culture conditions and culture medium components of the Lactobacillus caseis-03 were optimized by means of single factor and orthogonal experiments. The results showed that Lactobacillus caseis-03 was anaerobic bacteria；the optimum culture conditions were as follows: initial pH 6.5, 35 ℃, inoculation 1.0%, static cultured in improved MRS medium for 24 h; the optimum culture medium components were as follows: peptone 12 g, yeast extract 6 g, beef extract 6 g, glucose 18 g, sodium acetate 5 g, C6H17N3O7 2.15 g, Tween 80 1 mL, MgSO4·7H2O 0.58 g, MnSO4·4H2O 0.05 g, K2HPO4 2 g, H2O 1000 mL. D610 nm value could reach 1.832, viable bacteria counts could reach 3.22×1010 CFU/mL in the optimum culture medium and conditions. |
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| Keywords: | Lactobacillus caseis-03 culture conditions culture medium components optimization D610 nm value viable bacteria counts |
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