| 鸭出血性卵巢炎病毒RT-PCR检测方法的建立 |
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| 引用本文： | 万春和,施少华,程龙飞,陈红梅,傅光华,彭春香,林芳,林建生,黄瑜.鸭出血性卵巢炎病毒RT-PCR检测方法的建立[J].福建农业学报,2011,26(1):10-12. |
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| 作者姓名： | 万春和 施少华 程龙飞 陈红梅 傅光华 彭春香 林芳 林建生 黄瑜 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福建,福州,350013 |
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| 基金项目： | "十二五"国家水禽产业技术体系，科技部科技人员服务企业行动项目，福建省星火科技计划项目，福建省农业科学院科技创新团队建设基金 |
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| 摘 要： | 参照(GenBank上登录的黄病毒科黄病毒属Ns5基因片段序列设计引物,建立能检测引起鸭出血性卵巢炎病毒的RT-PCR方法.该办法能从鸭出血性卵巢炎病毒扩增出1条约470 bp的特异性片段,从H9亚型禽流感病毒、鸭呼肠孤病毒、鸭瘟病毒、鸭肝炎病毒、鸭源禽Ⅰ型副粘病毒均不能扩增出目的片段;敏感性试验显示建立的RT-PCR...
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| 关 键 词： | 鸭出血性卵巢炎 黄病毒 RT-PCR |
Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks |
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| WAN Chun-he,SHI Shao-hua,CHENG Long-fei,CHEN Hong-mei,FU Guang-hua,PENG Chun-xiang,LIN Fang,LIN Jian-sheng,HUANG Yu.Establishment of RT-PCR for detecting duck hemorrhagic ovaritis causing abrupt egg-laying reduction in ducks[J].Fujian Journal of Agricultural Sciences,2011,26(1):10-12. |
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| Authors: | WAN Chun-he SHI Shao-hua CHENG Long-fei CHEN Hong-mei FU Guang-hua PENG Chun-xiang LIN Fang LIN Jian-sheng HUANG Yu |
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| Institution: | (Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China) |
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| Abstract: | A pair of primers based on NS5 gene sequences of different strains of genus Flavivirus was designed for amplification of 470 bp fragments.No positive target fragment was available from H9 subtype influenza virus,Muscovy duck reovirus,duck plague,duck hepatitis virus(DHV-Ⅰ) or duck-origin avian paramyxovirus typeⅠ.The detection limit reached 20 pg under the optimized conditions.The results showed that the RT-PCR was sensitive,specific and applicable for rapid laboratory diagnosis and epidemiologic surveillance. |
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| Keywords: | RT-PCR |
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