一种新型根系分泌物收集装置与收集方法的介绍

根系分泌物在养分活化、改善环境胁迫方面具有重要作用,很多科技工作者对根系分泌物的研究表现出极大兴趣,取得了一系列进展。但土壤栽培条件下,根系分泌物收集是一个难点。本文介绍了一种新型根系分泌物的收集装置与收集方法。该装置由根系生长箱和分泌物收集箱组成,植物在生长箱土壤中生长,通过定向引导作用,根系从生长箱穿过琼脂层进入收集箱中生长,待收集箱内积累一定根系后,通过淋洗收集箱内的介质,实现根系分泌物收集。研究发现,利用该装置收集分泌物,植物总根尖数的90%分布在收集箱。外源有机酸加样回收率可达70%以上。土壤栽培条件下,随生长时期延长,大豆有机酸分泌量逐渐增加,苹果酸分泌量高于柠檬酸。而且土壤栽培条件下大豆柠檬酸和苹果酸分泌量是溶液栽培时的11.4倍和6.7倍。上述研究表明,该装置可以用于土壤栽培条件下根系有机酸的分泌研究。

A new type of device and method for collecting plant root exudates

As root exudates play an important role in mobilization of soil nutrients and mitigation of environmental stress, many scientists show great interests in root exudates, and have achieved some progresses in the research of those substances. However, it is still not an easy job to collect root exudates from plants growing in soil. Here, a new type of device and method is introduced for collecting root exudates from plants growing in soil. The device consists of a culture box and a collection box. During growth the plant in the culture box, affected by the oriented guidance technique, extends its roots through the agar medium into the collection box. When a certain amount of plant roots develop in the collection box, the medium in the box is leached for collection of root exudates. In this experiment, soybean was planted and cultured for 3 weeks. By then, more than 90% of the total root tips and 30% of the total root biomass were distributed in the collection box. In-situ recovery rate of organic acids reached over 70% in this system, it was found that secretion of organic acids from the roots of soybean under soil culture increased with its growth period. Soybean roots secreted more malate than citrate. Secretion of citrate and malate under soil culture were 11.4 and 6.7 times as much as under solution culture, respectively. The above findings suggest that this device is applicable to collecting organic acids secreted from plant roots under soil culture.