| Mineirao柱花草MSRA基因的克隆及其植物表达载体的构建 |
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| 引用本文： | 吴成贡,申建斌,田维敏,蒋昌顺.Mineirao柱花草MSRA基因的克隆及其植物表达载体的构建[J].热带作物学报,2008,29(4):430-434. |
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| 作者姓名： | 吴成贡 申建斌 田维敏 蒋昌顺 |
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| 作者单位： | 1. 中国热带农业科学院农业部热带作物栽培生理学重点开放实验室,海南,儋州,571737;海南大学农学院,海南,儋州,571737
2. 中国热带农业科学院农业部热带作物栽培生理学重点开放实验室,海南,儋州,571737 |
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| 基金项目： | 国家自然科学基金，华南热带农业大学校科研和教改项目，热带作物栽培生理学重点实验室开放课题基金 |
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| 摘 要： | 根据抗病柱花草中的1个AFLP特异片段SG2950-1设计引物,利用RACE技术从Mineirao柱花草中克隆到一个MSRA基因:经测序比对,该序列与细胞色素氧化酶系基因有较高的相似性.构建了植物表达载体,通过冻融法将该载体质粒转入根癌农杆菌LBA4404,为进行该基因的功能分析奠定了基础.
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| 关 键 词： | Mineirao柱花草 MSRA基因 植物表达载体 |
Cloning of MSRA Gene from Stylosanthes guianensis cv. Mineirao and Construction of Its Plant Expression Vector |
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| Wu Chenggong,Shen Jianbin,Tian Weimin and Jiang Changshun.Cloning of MSRA Gene from Stylosanthes guianensis cv. Mineirao and Construction of Its Plant Expression Vector[J].Chinese Journal of Tropical Crops,2008,29(4):430-434. |
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| Authors: | Wu Chenggong Shen Jianbin Tian Weimin and Jiang Changshun |
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| Abstract: | MSRA gene was cloned by using the method of rapid amplification of cDNA end (RACE) with the primer based on an AFLP special fragment designed. The sequencing showed that the sequence of this fragment shared high similarity with that of Cytochrome Oxidase genes. The MSRA gene was cloned into plant expression vector, and the recombination plasmids were then transformed into Agrobacterium tumefaciens LBA4404 by using the freezing-melting transformation method, which facilitates Agrobacterium mediated transformation of MSRA gene into plant. |
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| Keywords: | Stylosanthes guianensis cv Mineirao MSRA gene transformation vector |
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