吉林省主推中晚熟期玉米品种遗传多样性分析（摘要）

[目的]应用SSR分子标记研究吉林省主推中晚熟期玉米品种的遗传多样性。[方法]通过10对引物对供试材料进行扩增,并用聚丙烯凝胶电泳进行检测。[结果]10对SSR引物在供试材料中共检测出110个等位基因变异,每对SSR引物检测出的等位基因数为4～17个,平均每个位点11个,每个SSR位点的PIC变化范围在0.63～0.93之间,平均0.842。43个玉米杂交种之间的遗传相似系数变化范围为0.664～0.918,平均为0.774,其中样本间遗传相似系数在0.85以上的共有30个。结合聚类分析和各品种的谱系来源,结果表明43份吉林省主推中晚熟期玉米品种的遗传相似性相对较大。[结论]吉林省中晚熟期种质资源相对狭窄,有待于进一步引种创新。

Genetic Diversity Analysis of Dominant Middle-late Mature Maize Varieties in Jilin Province

[Objective] The aim was to study the genetic diversity of dominant middle-late mature maize hybrids in Jilin Province by the use of Simple sequence repeats （SSRs） molecular mark.[Method] PCR amplification was carried out based on ten core SSR primers and the amplification products were detected through sodium dodecyl sulfate polyacrylamide gel electrophoresis.[Result] Ten core SSR primers gave clear and stable profiles amplified in samples of 43 maize hybrids.A total of 110 polymorphic amplified fragments were produced by 10 primers.The average number of allele per SSR locus was 11 which varied from 4 to 17.The polymorphism information content （PIC） for the SSR loci ranged from 0.63 to 0.93 with an average of 0.842.Genetic similarities among the 43 maize hybrids varied from 0.664 to 0.918 with an average of 0.774 and those among 30 maize hybrids were greater than 0.85.Combined with the cluster analysis and pedigree origin of 43 maize hybrids,it was found that there were more genetic similarities among the 43 maize hybrids.[Conclusion] The germplasm resources of middle-late mature maize hybrids in Jilin Province were comparatively narrow and waited for innovation.