| 浙江省草莓炭疽病菌鉴定及生物学特性研究 |
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| 引用本文： | 任海英,蒋桂华,方丽,张豫超,杨肖芳,苗立祥,王汉荣. 浙江省草莓炭疽病菌鉴定及生物学特性研究[J]. 浙江农业学报, 2011, 23(5): 0 |
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| 作者姓名： | 任海英 蒋桂华 方丽 张豫超 杨肖芳 苗立祥 王汉荣 |
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| 作者单位： | 1.浙江省农业科学院 植物保护与微生物研究所, 浙江 杭州 310021；2浙江省农业科学院 园艺研究所,浙江 杭州 310021 |
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| 基金项目： | 浙江省科技厅重点项目(2009C12073) |
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| 摘 要： | 对分离自浙江各地的草莓炭疽病菌进行形态特征、培养性状等生物学特性研究并测定ITS序列,结果表明：浙江各地引起草莓炭疽病的优势菌株为Colletotrichum gloeosporioides,该病原菌最适在PDA和PSA培养基上生长,菌丝生长和孢子萌发的最适温度为25～30℃,培养基的最适pH值为6.0～7.0,但孢子在pH值6.0时萌发率最高。以蔗糖和果糖为碳源,硝酸钾为氮源时生长速率最快。
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| 关 键 词： | 草莓 炭疽病菌 病原鉴定 生物学 |
Identification and characterization of the pathogen of strawberry anthracnose in Zhejiang Province |
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| REN hai-Ying,JIANG Gui-hua,FANG Li,ZHANG Yu-chao,YANG Xiao-fang,MIAO Li-xiang,WANG han-rong. Identification and characterization of the pathogen of strawberry anthracnose in Zhejiang Province[J]. Acta Agriculturae Zhejiangensis, 2011, 23(5): 0 |
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| Authors: | REN hai-Ying JIANG Gui-hua FANG Li ZHANG Yu-chao YANG Xiao-fang MIAO Li-xiang WANG han-rong |
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| Affiliation: | 1Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China；2Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | Morphologic traits, cultural characteristics and rDNA ITS sequences were studied for the pathogens of strawberry anthracnoses collected in Zhejiang Province. The results showed that Colletotrichum gloeosporioides was the predominant pathogen causing the disease in Zhejiang. PDA and PSA culture media were suitable for the growth of C. gloeosporioides and the optimal temperature was 25-30℃. It was found that spore germination rate was the highest in pH 6.0, however, the suitable pH to maintain mycelium growth was 6.0-7.0. The growth rate of mycelium was optimized using sucrose and fructose as carbon source and potassium nitrate as nitrogen source． |
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| Keywords: | strawberry anthracnose pathogen identification biology |
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