| 高效液相色谱法测定水稻穗部的GA3含量 |
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| 引用本文： | 陈胜军,王艳丽,张震,姜华,柴荣耀,毛雪琴,邱海萍,王教瑜,杜新法.高效液相色谱法测定水稻穗部的GA3含量[J].浙江农业学报,2011,23(6). |
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| 作者姓名： | 陈胜军 王艳丽 张震 姜华 柴荣耀 毛雪琴 邱海萍 王教瑜 杜新法 |
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| 作者单位： | 1. 浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地,浙江省农业科学院植物保护与微生物研究所,浙江杭州310021;杭州师范大学生命与环境科学学院,浙江杭州310036
2. 浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地,浙江省农业科学院植物保护与微生物研究所,浙江杭州310021 |
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| 基金项目： | 国家自然科学基金资助项目(3090093330970082); 浙江省重大科技攻关项目(2007C12905) |
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| 摘 要： | 以水稻穗部为材料,探索内源赤霉酸( Gibberellin A3,GA3)的提取和纯化方法,利用高效液相色谱法建立分析测定方法.针对水稻样品富含色素和多酚类等杂质的特点,用氯仿去除色素,然后采用三步萃取法纯化GA3.在以往用高效液相色谱法分析植物内源激素的研究基础上,试验对色谱条件进行了改进.研究发现,最优色谱条件包括紫外检测器在254 nm波长下进行检测,以乙腈-水-乙酸(体积比为20∶79∶1)溶液为流动相,流速为1.0 mL·min-1等条件下峰形良好且保留时间稳定.结果表明,按照本试验流程,样品的添标回收率在80％以上,且相对标准偏差小于3％,较高的回收率和精密度表明本试验流程可以准确地分析测定水稻穗部GA3.
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| 关 键 词： | 水稻 穗部 高效液相色谱 赤霉酸 |
Determination of gibberellin A_3 in rice panicle by high-performance liquid chromatography |
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| CHEN Sheng-jun
,
WANG Yan-li
,
ZHANG Zhen
,
JIANG Hua
,
CHAI Rong-yao
,
MAO Xue-qin
,
QIU Hai-ping
,
WANG Jiao-yu
,
DU Xin-fa.Determination of gibberellin A_3 in rice panicle by high-performance liquid chromatography[J].Acta Agriculturae Zhejiangensis,2011,23(6). |
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| Authors: | CHEN Sheng-jun WANG Yan-li ZHANG Zhen JIANG Hua CHAI Rong-yao MAO Xue-qin QIU Hai-ping WANG Jiao-yu DU Xin-fa |
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| Institution: | CHEN Sheng-jun1,2,WANG Yan-li1,ZHANG Zhen1,JIANG Hua1,CHAI Rong-yao1,MAO Xue-qin1,QIU Hai-ping1,WANG Jiao-yu1,DU Xin-fa1,*(1State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,Institute of Plant Protection Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China,2College of Life and Environment Sciences,Hangzhou Normal University,Hangzhou 310036,China) |
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| Abstract: | This paper aims to find out a suitable method for extracting,purifying and analyzing endogenous gibberellin A3 of rice panicle by high-performance liquid chromatography method.To deal with the existence of large amount of pigments,polyphenols and other impurities,pigment was eliminated by chloroform and then gibberellin A3 was purified by liquid-liquid extraction of three steps.On the basis of former reports,the high-performance liquid chromatographic conditions for analyzing endogenous hormone of plant wer... |
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| Keywords: | rice panicle high-performance liquid chromatography gibberellin A3 |
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