白首乌的组织培养条件的优化

以白首乌茎段、叶片、茎尖和带腋芽茎段为外植体,进行离体培养植株再生研究,结果表明：适合初代诱导的培养基为：MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1,诱导率可达100%；最佳外植体是带腋芽茎段；芽苗增殖的最佳培养基为：MS+KT 1.5 mg·L-1+NAA 0.05 mg·L-1,增殖系数为5.6；生根培养基为：1/2 MS+NAA 0.5 mg·L-1,生根率为93.3%。

Tissue culture and plant regeneration of Cynanchum auriculatum

Tender stem, leaves, shoot apex ,and stem with axillary bud of Cynanchum auriculatum were used as explants in tissue culture and plant regeneration had been studied. The results indicated that the best explants was stem with axillary bud. The most appropriate medium for the original induction was MS+6-BA 1.0 mg ·L-1+NAA 0.1 mg ·L-1,with the induction rate of 100%. The optimum medium for multiplication of buds was MS+KT 1.5 mg ·L-1+NAA 0.05 mg ·L-1,and the coefficient of multiplication was 5.6. The medium for rooting was 1/2 MS+NAA 0.5 mg ·L-1 and the rooting rate reached 93.3%．