| 半夏的组织培养与快繁试验 |
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| 引用本文： | 杨玉珍,王西雨,胡如善,孙廷. 半夏的组织培养与快繁试验[J]. 山东农业科学, 2010, 0(1): 10-11 |
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| 作者姓名： | 杨玉珍 王西雨 胡如善 孙廷 |
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| 作者单位： | 1. 南阳农校植物组织培养中心,河南,南阳,473003
2. 南阳市园林局,河南,南阳,473003 |
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| 基金项目： | 河南省南阳市重点科技攻关项目“植物脱毒及快繁技术研究与推广”(编号:2001ZD003) |
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| 摘 要： | 以传统中药半夏的小块茎为外植体进行组织培养，对适合不定芽分化增殖、生根的培养基进行了研究。结果表明，利于愈伤组织诱导的培养基为MS＋6-BA2．0mg／L＋NAA0．2mg／L，利于不定芽增殖的培养基为MS＋6-BA2．0mg／L＋NAA0．5mg／L，利于生根的培养基为MS＋NAA0．5mg／L。试验表明，应用本项组织培养技术大批量生产优质半夏种苗是可行的。
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| 关 键 词： | 半夏 不定芽诱导 增殖 组织培养 快速繁殖 |
Tissue Culture and Rapid Propagation of Pinellia ternata |
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| YANG Yu-zhen,WANG Xi-yu,HU Ru-shan,SUN Ting. Tissue Culture and Rapid Propagation of Pinellia ternata[J]. Shandong Agricultural Sciences, 2010, 0(1): 10-11 |
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| Authors: | YANG Yu-zhen WANG Xi-yu HU Ru-shan SUN Ting |
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| Affiliation: | 1.Center of Plant Tissue Culture;Nanyang Agricultural School;Nanyang 473003;China;2.Nanyang Garden Bureau;China |
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| Abstract: | The small tubers from Pinellia ternata were used as explants for tissue culture.The culture media for multiplication of adventitious bud and rooting were studied in this paper.The results indicated that the suitable culture medium for inducing calli was MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L,while that for multiplication of adventitious bud was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,for rooting was MS + NAA 0.5 mg/L.And it was practicable to produce a large number of high quality Pinellia ternata plantlets by this tissue... |
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| Keywords: | Pinellia ternata Adventitious bud induction Multiplication Tissue culture Rapid propagation |
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