| 牡丹花瓣脂氧合酶测定方法的研究 |
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| 引用本文： | 李铭韧,史国安.牡丹花瓣脂氧合酶测定方法的研究[J].山东农业科学,2010(9). |
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| 作者姓名： | 李铭韧 史国安 |
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| 作者单位： | 河南科技大学农学院,河南,洛阳,471003 |
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| 基金项目： | 国家自然科学基金项目，河南省自然科学基金项目，洛阳市重点实验室专项 |
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| 摘 要： | 采用紫外分光光度法,以亚油酸钠为反应底物,研究了牡丹花瓣脂氧合酶(LOX)的酶学特性并建立了其测定体系。结果表明,牡丹花瓣脂氧合酶反应的最适pH值为4.5,最适温度为45℃,最适底物浓度为0.594 mmol/L。该酶有着较好的热稳定性,90℃保温1 h后仍保持较高活性。
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| 关 键 词： | 牡丹花瓣 脂氧合酶(LOX) 测定方法 |
Determination Method of Lipoxygenase Activity in Peony Petal |
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| LI Ming-ren,SHI Guo-an.Determination Method of Lipoxygenase Activity in Peony Petal[J].Shandong Agricultural Sciences,2010(9). |
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| Authors: | LI Ming-ren SHI Guo-an |
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| Institution: | LI Ming-ren,SHI Guo-an(College of Agronomy,Henan University of Science and Technology,Luoyang 471003,China) |
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| Abstract: | The enzymatic characteristics of lipoxygenase(LOX) in peony petal were studied by UV-spectrophotometry with the sodium linoleate as the reaction substrate,then the determination system was built.The results showed that the optimum pH value for the reaction was 4.5;the optimum temperature was 45℃;the optimum substrate concentration was 0.594 mmol/L.The enzyme had a good thermal stability.The enzyme activity kept higher in 90℃ for one hour. |
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| Keywords: | Peony petal Lipoxygenase Determination method |
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