| 猕猴桃雌雄性别的AFLP鉴别中DNA模板的制备 |
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| 引用本文： | 张潞生,李传友,贾建航,李绍华,王斌,肖兴国. 猕猴桃雌雄性别的AFLP鉴别中DNA模板的制备[J]. 果树学报, 1999, 16(3): 171-175 |
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| 作者姓名： | 张潞生 李传友 贾建航 李绍华 王斌 肖兴国 |
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| 作者单位： | 1. 中国农业大学,北京,100094
2. 中国科学院遗传研究所 |
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| 摘 要： | 基因组DNA的成功制备和避免降解是AFLP成功的关键。描述了猕猴桃AFLP研究中的DNA模板制备方法，尤其是DNA的提取和纯化，以及基因组DNA的酶切中常见的问题及其相应的解决措施。应用这些方法得到了猕猴桃清晰的AFLP指纹图谱。
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| 关 键 词： | 猕猴桃 AFLP DNA模板 |
Making DNA Template in the Study of Sex Determination in Actinidia by AFLP |
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| Zhang Lusheng,Li Chuanyou,Jia Jianhang,Li Shaohua,Wang Bin,Xiao Xingguo. Making DNA Template in the Study of Sex Determination in Actinidia by AFLP[J]. Journal of Fruit Science, 1999, 16(3): 171-175 |
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| Authors: | Zhang Lusheng Li Chuanyou Jia Jianhang Li Shaohua Wang Bin Xiao Xingguo |
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| Abstract: | The successful preparation of DNA template is the key to the AFLP marker of sex identification in Actinidia. In this paper we provided some efficient methods for the DNA template preparation,in particularly in DNA extraction, DNA purification and DNA digestion. Using these methods we got very clear AFLP pattern in the AFLP study of sex identification in Actinidia. |
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| Keywords: | AFLP |
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