首页 | 本学科首页   官方微博 | 高级检索  
     检索      

利用微卫星DNA标记进行杂交水稻种子纯度鉴定的研究
引用本文:詹庆才.利用微卫星DNA标记进行杂交水稻种子纯度鉴定的研究[J].杂交水稻,2002,17(5):46-50.
作者姓名:詹庆才
作者单位:湖南省水稻研究所,湖南,长沙,410125
基金项目:湖南省科委、计委资助项目
摘    要:利用微卫星分子标记,对湖南目前推广的6个杂交稻组合及其亲本之间的多态性进行了研究。在已筛选的178对引物中,有52对能在一个或多个组合中显示稳定的多态性,杂种表现为父母本互补带型。不同组合间多态性的比例具有差异。用表现多态性的两对引物分别对掺假的威优46和金优207进行纯度鉴定,都能有效地将掺入组合中的假种子区分开。该方法具有准确可靠、多态性高、稳定性好、易于操作的特点。

关 键 词:微卫星DNA标记  种子纯度  杂交水稻  纯度鉴定
文章编号:1005-3956(2002)05-0046-05
修稿时间:2002年1月30日

Studies on Identification of Purity and Factuality of Hybrid Rice Seeds Using Microsatellite Marker
ZHAN Qing,cai.Studies on Identification of Purity and Factuality of Hybrid Rice Seeds Using Microsatellite Marker[J].Hybrid Rice,2002,17(5):46-50.
Authors:ZHAN Qing  cai
Abstract:Six hybrid rice combinations and their parents were used to screen their polymorphic patterns using microsatellite markers. Out of 178 primer pairs, 52 pairs showed polymorphic patterns in one or more of the tested combinations. The F 1 hybrids showed the complementary patterns of their parents.Using two primer pairs of markers, we tested the purity and factuality of Weiyou 46 and Jinyou 207 F 1 seeds, which were purposely adulterated with the seeds of their male and female parents. By combining the rapid, simple and low cost method on DNA extraction, we found it was easy to identify the purity and factuality of hybrid seeds with the microsatellite marker method.
Keywords:hybrid rice  microsatellite marker  purity identification
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号