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| 贵州48个玉米杂交种及其亲本SSR指纹图谱的构建与分析 | |
| 引用本文： | 王伟,杨文鹏,张文龙,祝云芳,潘敏娜,沈建华,戴保威.贵州48个玉米杂交种及其亲本SSR指纹图谱的构建与分析[J].贵州农业科学,2009,37(11). |
| 作者姓名： | 王伟 杨文鹏 张文龙 祝云芳 潘敏娜 沈建华 戴保威 |
| 作者单位： | 1. 贵州省旱粮研究所,贵州,贵阳550006;贵州省农业生物技术重点实验室,贵州,贵阳550006;贵州大学,农学院,贵州,贵阳,550025 2. 贵州省旱粮研究所,贵州,贵阳550006;贵州省农业生物技术重点实验室,贵州,贵阳550006 3. 贵州省种子总站,贵州,贵阳,550001 4. 贵州省旱粮研究所,贵州,贵阳550006 5. 贵州大学,农学院,贵州,贵阳,550025 |
| 基金项目： | 贵州省科技计划项目"贵州省农作物分子标记应用技术研究"，国家科技支撑计划项目"喀斯特山区特有作物优异基因资源发掘与转基因安全关键技术研究与应用" |
| 摘 要： | 从北京市农林科学院筛选出的100对玉米SSR核心引物中选取20对,利用SSR分子标记技术,构建了贵州省2000年以来审定的48个玉米杂交种及其亲本的数字化指纹图谱.图谱构建所用的20个SSR标记每个位点的等位基因数平均为5.3个,多态信息量平均为0.66,标记索引系数平均为3.58,多态性丰富.图谱中,来自不同地区或不同育种家的同名自交系,有的指纹图谱较一致,有的有差异,有的扩增片段大小不同,有的扩增片段数目也不同;杂交种的谱带表现为偏母型、偏父型、杂种型和双亲互补型4种类型.经验证,建立图谱时所用的材料符合最小品种内变异的原则,表明所建立的图谱是准确的.该图谱对于玉米品种及其亲本的纯度鉴定、真实性鉴定以及维护育种家的权益有一定的应用价值. |
| 关 键 词： | DNA指纹 图谱 玉米 杂交种 贵州 |
Establishment and Analysis on SSR Fingerprint Mapping of 48 Hybrid Maize Varieties and Their Parents in Guizhou |
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| WANG Wei,YANG Wen-peng,ZHANG Wen-long,ZHU Yun-fang,PAN Ming-na,SHEN Jian-hua,DAI Bao-wei.Establishment and Analysis on SSR Fingerprint Mapping of 48 Hybrid Maize Varieties and Their Parents in Guizhou[J].Guizhou Agricultural Sciences,2009,37(11). | |
| Authors: | WANG Wei YANG Wen-peng ZHANG Wen-long ZHU Yun-fang PAN Ming-na SHEN Jian-hua DAI Bao-wei |
| Institution: | WANG Wei1,2,3,YANG Wen-peng1,2*,ZHANG Wen-long4,ZHU Yun-fang1,PAN Ming-na1,SHEN Jian-hua1,DAI Bao-wei3(1.Guizhou Institute of Upland Crops,Guiyang,Guizhou 550006,2.Guizhou Key Laboratory of Agricultural Biotechnology,3.College of Agriculture,Guizhou University,Guizhou 560025,4.Guizhou General Seed Station,Guizhou 550001,China) |
| Abstract: | Twenty pairs of SSR primers from 100 pairs of core SSR maize primers screened by Beijing Academy of Agricultural and Forestry Sciences were selected to establish SSR fingerprint mapping of 48 hybrid maize varieties and their parents approved by Guizhou Crops Variety Examination Committee since 2000.The average alleles,polymorphic information content and marker index coefficient of 20 SSR markers are 5.3,0.66 and 3.58 respectively.There is the same or different fingerprint mapping and different size or amoun... |
| Keywords: | SSR SSR DNA fingerprint mapping maize hybrid Guizhou |
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