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| EDTA和金属离子对多聚半乳糖醛酸酶酶活力的影响及其动力学研究 | |
| 引用本文： | 刘小林,伍志权,卓怡,黄卓烈. EDTA和金属离子对多聚半乳糖醛酸酶酶活力的影响及其动力学研究[J]. 江西农业大学学报, 2010, 32(1) |
| 作者姓名： | 刘小林 伍志权 卓怡 黄卓烈 |
| 作者单位： | 1. 江西宜春学院,生命科学学院,江西,宜春,336000;华南农业大学,生命科学学院,广东,广州,510642 2. 佛山市中南农业科技有限公司检测中心,广东,佛山,528200 3. 华南农业大学,生命科学学院,广东,广州,510642 |
| 基金项目： | 国家自然科学基金资助项目(10074016) |
| 摘 要： | 实验以EDTA和不同金属离子为效应物,果胶为反应底物,研究不同浓度的EDTA,Na+,K+,Ca2+,Cu2+,Zn2+,Mn2+,Fe2+,Fe3+,Ag+和Ba2+对多聚半乳糖醛酸酶(PG)催化活性的影响。结果表明,一定浓度的Cu2+,Zn2+,Mn2+,Fe2+和Fe3+对PG有激活作用,对PG产生最大激活作用的浓度分别为0.3mmol/L,0.4mmol/L,0.3mmol/L,0.3mmol/L和0.6mmol/L,EDTA,Ca2+,Ag+和Ba2+对PG有抑制作用,Na+和K+对PG活性没有影响。动力学研究表明,0.3mmol/LNa+和K+处理后,PG的Km和Vmax基本没有发生变化,2mmol/LEDTA和0.3mmol/LCa2+处理后,PG的Km和Vmax均下降,0.3mmol/LCu2+,Zn2+,Mn2+,Fe2+,Fe3+处理后,PG的Km和Vmax都上升,0.3mmol/LAg+和Ba2+处理后,PG的Km上升,Vmax下降。 |
| 关 键 词： | 多聚半乳糖醛酸酶 金属离子 动力学 |
A Study on the Effects of EDTA and Different Metal Ions on Enzyme Specific Activity of Polygalacturonase and Its Kinetics Analysis |
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| LIU Xiao-lin,WU Zhi-quan,ZHUO Yi,HUANG Zhuo-lie. A Study on the Effects of EDTA and Different Metal Ions on Enzyme Specific Activity of Polygalacturonase and Its Kinetics Analysis[J]. Acta Agriculturae Universitis Jiangxiensis, 2010, 32(1) | |
| Authors: | LIU Xiao-lin WU Zhi-quan ZHUO Yi HUANG Zhuo-lie |
| Affiliation: | 1.College of Life Science/a>;Yichun University/a>;Yichun 336000/a>;China/a>;2.Test Center/a>;Foshan Zhongnan Agricultural Technology co Ltd./a>;Foshan 528200/a>;3.College of Life Science/a>;South China Agricultural University/a>;Guangzhou 510642/a>;China |
| Abstract: | In this experiment,EDTA and different metal ions were used as effectors,and pectin as substrate in order to study the the effects of EDTA and different metal ions on enzyme specific activity of polyga-lacturonase(PG).The metal ions included Na+,K+,Ca2+,Cu2+,Zn2+,Mn2+,Fe2+,Fe3+,Ag+ and Ba2+.The result showed that the enzyme specific activity of PG increased after the treatment with certain concentrations of Cu2+,Zn2+,Mn2+,Fe2+ and Fe3+.The concentration which caused the max activation of PG of Cu2+,Zn2+,Mn2+... |
| Keywords: | polygalacturonase metal ion kinetics analysis |
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