| EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响 |
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| 引用本文： | 陈勋,于海宁,沈生荣.EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响[J].茶叶科学,2006,26(3):219-224. |
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| 作者姓名： | 陈勋 于海宁 沈生荣 |
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| 作者单位： | 1. 浙江大学茶叶研究所, 浙江 杭州 310029;2. 浙江工业大学药学院,浙江 杭州 310032 |
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| 基金项目： | 中国科学院资助项目;中国博士后科学基金 |
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| 摘 要： | 本文采用MTT法研究了EGCG以及EGCG与锌离子相互作用对前列腺癌细胞PC-3生长的影响;采用原子吸收光谱法(AAS)研究了锌离子及EGCG共存对PC-3细胞锌、镉离子吸收的影响;利用HPLC测定了锌离子对EGCG在PBS缓冲液与正辛醇,水与正辛醇体系中分配系数的影响。结果表明,EGCG能抑制前列腺癌细胞生长,细胞存活率呈时间和剂量依赖性;锌离子能抑制PC-3细胞的生长,促进EGCG对PC-3细胞生长的抑制作用,呈时间和剂量依赖性;同时,锌离子能抑制PC-3细胞对镉离子的吸收,而EGCG有增强效应。
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| 关 键 词： | 儿茶素 Zn2+ Cd2+ PC-3细胞 |
| 文章编号： | 1000-369X（2006）03-219-06 |
| 收稿时间： | 2006-01-19 |
| 修稿时间： | 2006-05-27 |
Effect of Interactions Between EGCG and Zn2+ on the Growth of Prostate Cancer PC-3 Cells |
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| CHEN Xun,YU Hai-ning,SHEN Sheng-rong.Effect of Interactions Between EGCG and Zn2+ on the Growth of Prostate Cancer PC-3 Cells[J].Journal of Tea Science,2006,26(3):219-224. |
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| Authors: | CHEN Xun YU Hai-ning SHEN Sheng-rong |
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| Institution: | 1. Institute of Tea Sciences, Zhejiang University, Hangzhou 310029, China;2. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310032, China |
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| Abstract: | In the present paper,effects of EGCG and interactions of EGCG with Zn2 on the growth of androgen-insensitive human prostate cancer cells(PC-3) were investigated by MTT assay.Effects of EGCG and Zn2 on absorption of zinc and cadmium in PC-3 cells were detected by Flame Atomic Absorption Spectrometry(AAS) assay;and also the changes of Zn2 on the partition coefficients of EGCG in n-octanol/PBS or n-octanol / water system were determined by HPLC technique.EGCG was screened for growth inhibition of PC-3 cells in dose-and time-dependent manners.Both Zn 2 and EGCG decreased viability of PC-3 cells in dose-and time-dependent relations,and Zn2 ameliorated the effect of EGCG on the growth of PC-3 cells.Also,Zn2 decreased the absorption of PC-3 cells on Cd2 ,and EGCG could enhance the effect as well. |
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| Keywords: | Zn2 Cd2 |
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