宁夏苦马豆内生真菌的分离与鉴定

为研究苦马豆（Swainsona salsula）中内生真菌的种属分类，对采自宁夏的苦马豆内生真菌进行分离培养和形态观察，对分离菌株的ITS序列进行扩增、测序和系统发育分析。从宁夏苦马豆植株的叶和茎中共分离出4个真菌菌株，编号分别为SS_NXB1、SS_NXF1、SS_NXG2、SS_NXA1，因植株未表现出任何病害症状，故确定其为内生真菌。根据形态特征和ITS序列，确定前3株真菌为枝顶孢属（Acremonium）真菌。因菌株SS_NXA1未产生分生孢子及GenBank数据库中缺少同源菌株的分类信息而未确定其分类地位。

Isolation and identification of fungal endophytes from Swainsona salsula grown in Ningxia

This study was performed to investigate the taxonomy of fungal endophytes from Swainsona salsula. The fungal endophytes were isolated from S.salsula collected from Ningxia and observed under the microscope. The ITS sequences of the fungal isolates were amplified and sequenced. Analysis of molecular phylogeny based on the fungal ITS sequences was conducted to help to determine the taxonomies of fungal isolates. Four strains, SS_NXB1, SS_NXF1, SS_NXG2 and SS_NXA1, were isolated from S.salsula in this report. The isolates were considered as fungal endophytes of S.salsula with no disease observed on the surface of the plant samples. The first three isolates, SS_NXB1, SS_NXF1 and SS_NXG2, were classified as Acremonium sp. based on the fungal morphology and ITS sequences. However, SS_NXA1 could not be made a taxonomic placement due to the lack of natural conidia and sufficient sequences that represented in GenBank sequence databases.