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| 地黄花叶病毒的分子检测 | |
| 引用本文： | 杨玲,张振臣,张荣意.地黄花叶病毒的分子检测[J].河南农业科学,2009(4). |
| 作者姓名： | 杨玲 张振臣 张荣意 |
| 作者单位： | 1. 海南大学,环境与植物保护学院,海南,海口,571737;河南省农业科学院,植物保护研究所,河南,郑州,450002 2. 河南省农业科学院,植物保护研究所,河南,郑州,450002 3. 海南大学,环境与植物保护学院,海南,海口,571737 |
| 摘 要： | 设计了能同时检测Re MV、TMV和ToMV的简并引物,利用RT-PCR的方法对我国不同地区的地黄病毒样品进行了检测。共克隆了25个病毒样品的外壳蛋白(CP)基因,序列测定结果表明,24个病毒样品均是Re MV,其相近病毒TMV未检测到,ToMV仅在一个野生地黄样品上检出,说明Re MV在我国栽培地黄和野生地黄上普遍存在。 |
| 关 键 词： | 地黄花叶病毒 分布 番茄花叶病毒 |
Molecule Detection of Rehmannia Mosaic Virus |
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| YANG Ling,ZHANG Zhen-chen,ZHANG Rong-yi.Molecule Detection of Rehmannia Mosaic Virus[J].Journal of Henan Agricultural Sciences,2009(4). | |
| Authors: | YANG Ling ZHANG Zhen-chen ZHANG Rong-yi |
| Institution: | 1.Environment and Plant Protection College;Hainan University;Haikou 571737;China;2.Institute of Plant Protection;Henan Academy of Agricultural Sciences;Zhengzhou 450002;China |
| Abstract: | Virus samples infected Rehmannia glutinosa in different regions were detected by using RT-PCR with the degenerate primers designed to detect Rehmannia mosaic virus(ReMV),Tobacco mosaic virus(TMV) and Tomato mosaic virus(ToMV)simultaneously.Coat protein(CP) genes of 25 virus samples were cloned and sequenced.The results indicate that the 24 virus samples were ReMV,TMV was not detected,the ToMV is detected only in one wild Rehmannia glutinosa sample.According to the above experiment,ReMV is the major virus in... |
| Keywords: | Rehmannia mosaic virus Distribute Tomato mosaic virus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |