| 分离培养兔子宫内膜细胞的鉴定及性激素对子宫内膜间质细胞形态的影响 |
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| 引用本文： | 陈秀荔,靳亚平,张彦明. 分离培养兔子宫内膜细胞的鉴定及性激素对子宫内膜间质细胞形态的影响[J]. 畜牧兽医学报, 2006, 37(11): 1226-1231 |
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| 作者姓名： | 陈秀荔 靳亚平 张彦明 |
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| 作者单位： | 西北农林科技大学,农业部家畜生殖内分泌与胚胎工程重点开放实验室,杨凌,712100 |
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| 基金项目： | 国家自然科学基金资助项目（39770544） |
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| 摘 要： | 为探讨子宫内膜细胞的形态学特点、分泌功能及性激素在子宫内膜细胞中的作用，采用不同的消化分离方法进行了孕早期家兔子宫内膜细胞的消化和分离培养试验。结果显示：子宫内膜主要包括两类细胞，基质细胞和上皮细胞，两类细胞分别在波形蛋白和细胞角蛋白免疫细胞化学染色中有阳性反应特点；在培养基中加入雌激素和／或孕酮刺激后，基质细胞形态发生变化，表现一定的蜕膜化现象。
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| 关 键 词： | 子宫内膜 免疫组织化学 雌激素 孕酮 蜕膜化 |
| 文章编号： | 0366-6964（2006）11-1226-06 |
| 收稿时间： | 2005-08-10 |
| 修稿时间： | 2005-08-10 |
The Identification of Separated and Cultured Rabbit Endometrial Cells and the Effect of Sex Hormone on Shape of Endometrial Stromal Cells |
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| CHEN Xiu-li,JIN Ya-ping,ZHANG Yan-ming. The Identification of Separated and Cultured Rabbit Endometrial Cells and the Effect of Sex Hormone on Shape of Endometrial Stromal Cells[J]. Chinese Journal of Animal and Veterinary Sciences, 2006, 37(11): 1226-1231 |
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| Authors: | CHEN Xiu-li JIN Ya-ping ZHANG Yan-ming |
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| Affiliation: | Key Laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology of Agriculture Ministry of China, Northwest Sci-Tech University of Agriculture and Forestry, Ya ngling 712100, China |
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| Abstract: | To study the morphological characteristics, the secretory capability of endometrial cells and the effect of sex hormone to endometrial cells, different digestion methods and isolation methods were used to digest and isolate endometrial cells from early pregnant rabbit. The results showed that endometrial cells include two types of cells, stromal cells and epithelial cells. Two types of cells were positive respectively with vimentin or cytokeratin by immunocytochemistry. When estradiol and/or progesterone were interfused in the medium, morphological characteristics of stromal cells changed, representing decidualization to some extent. |
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| Keywords: | endometrium immunoeytoehemistry estradiol progesterone deeidualization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
