| 人外周血CD4+ T细胞F-actin的超分辨SIM成像 |
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| 引用本文： | 任敏,张先恩,崔宗强,张吉斌.人外周血CD4+ T细胞F-actin的超分辨SIM成像[J].华中农业大学学报,2016,35(1):120-125. |
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| 作者姓名： | 任敏 张先恩 崔宗强 张吉斌 |
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| 作者单位： | 1. 华中农业大学农业微生物学国家重点实验室，武汉 430070; 中国科学院武汉病毒研究所，武汉 430070;2. 中国科学院武汉病毒研究所,武汉,430070;3. 华中农业大学农业微生物学国家重点实验室,武汉,430070 |
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| 基金项目： | 国家自然科学基金项目（31470269） |
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| 摘 要： | 通过合成lifeact序列,将其克隆到pEGFP-C1载体上,构建了pEGFP-C1-Lifeact质粒,转染原代CD4+T细胞,实现对CD4+T细胞F-actin的荧光标记,结合超分辨3D-SIM荧光成像技术,研究人外周血CD4+T细胞F-actin的精细结构,并对其动力学过程进行连续观察。结果表明,CD4+T细胞F-actin分布在细胞膜周围,处于解聚和聚合的动态平衡状态,与普通荧光显微镜相比,超分辩SIM成像F-actin结构更加清晰,分辨率提高2~4倍。
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| 关 键 词： | CD4 %2B T 细胞 人外周血 F-actin 超分辨 SIM 成像 |
Super-resolution structured illumination microscopy (SIM)imaging of F-actin in human peripheral blood CD4 + T cells |
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| Abstract: | To label the CD4 + T cells F-actin,the lifeact gene was synthesized and cloned into vec-tor pEGFP-C1,CD4 + T cells were then transfected with pEGFP-C1-Lifeact plasmid and imaged by the real time super-resolution 3D-SIM fluorescence microscopy.The results showed that F-actin is distribu-ted around the cellular membrane with steady state of depolymerization and polymerization.It was indi-cated that the spatial resolution of the fine SIM imaging of F-actin was two to four times higher than the wide field fluorescence microscope imaging. |
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| Keywords: | CD4+ T cells human peripheral blood F-actin super-resolution SIM imaging |
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