解钾菌解钾效率检测方法的比较 |
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引用本文: | 王珣珏,黄巧云,蔡鹏,陈雯莉. 解钾菌解钾效率检测方法的比较[J]. 华中农业大学学报, 2016, 35(1): 81-85 |
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作者姓名: | 王珣珏 黄巧云 蔡鹏 陈雯莉 |
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作者单位: | 华中农业大学农业微生物学国家重点实验室,武汉,430070 |
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基金项目: | 国家重点基础研究发展计划(“973”计划)项目(2015CB150504);教育部长江学者和创新团队发展计划项目(IRT1247);中央高校基本科研业务费专项(2662015PY016,2013PY111,2013PY136) |
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摘 要: | 以中慢生根瘤菌S-15和类芽胞杆菌S-17作为供试菌株,采用细胞破碎、NH4OAc浸提、H2O2溶液消煮及不作任何前处理等4种方法,利用火焰光度计检测解钾菌发酵液中K+含量,并计算解钾菌在培养基中的解钾效率。结果表明,配制的3种钾系列标准溶液所绘制的钾标准曲线较为接近,R2分别高达0.994 4、0.9997、0.999 8。采用H2O2消煮后所测得的K+浓度最高,2株解钾菌的解钾效率分别达到101.1%、125.1%,与其他处理组之间有显著性差异。用H2O2溶液处理所得到的解钾率更能真实反映解钾菌的解钾作用。
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关 键 词: | 解钾菌 解钾效率 钾离子 生物钾肥 前处理方法 检测方法 |
收稿时间: | 2015-09-12 |
Comparing different methods of detecting potassium solubilizing efficiency with potassium solubilizing bacteria |
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Abstract: | Using Mesorhizobium sp.S-15 and Paenibacillus sp.S-17 as experimental strains.After cell disruption,NH 4 OAc extraction,H 2 O 2 solution digestion or without any pre-treatment,K+ content in the fermentation broth of potassium solubilizing bacteria was determined with flame photometer.The ef-ficiency of K solubilizing by potassium solubilizing bacteria in the culture medium was calculated.The re-sults showed that the standard curves of the three kinds of K series of standard solutions were similar with the R 2 values of 0.994 4,0.999 7 and 0.999 8,respectively.After digested by H 2 O 2 solution,concen-tration of K+ was detected.The efficiency of two strains was 101.1% and 125.1%,significantly higher than that of other groups.The efficiency of potassium solubilizing detected after H 2 O 2 digestion well re-flected potassium solubilization of bacteria. |
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Keywords: | potassium solubilizing bacteria efficiency of potassium solubilizing K+ bio-potassium fertilizer pre-treatment methods detection method |
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