Construction of a multiple targeting RNAi plasmid that inhibits target gene expression and FMDV replication in BHK-21 cells and suckling mice |
| |
Authors: | Wei Cong Shaoqiang Cui Jiulian Chen Xiaoping Zuo Yonggan Lu Weiyao Yan Zhaoxin Zheng |
| |
Institution: | (1) State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai, 200433, People’s Republic of China;(2) Bio-pharmacy, Jinyu Group Co. LTD, Hohhot, Inner Mongolia , 010020, People’s Republic of China; |
| |
Abstract: | Foot-and-mouth disease (FMD) is a highly contagious disease that afflicts cloven-hoofed animals. The etiological agent of
FMD is foot-and-mouth disease virus (FMDV). The VP1 gene of FMDV is essential during the life cycle of the virus and plays
a key role in the attachment of the virus to susceptible cells. We constructed a plasmid, pCWN11, that expresses siRNAs multiple-targeting
the VP1 genes of FMDV. We evaluated the gene silencing efficiency of the plasmid using an enhanced green fluorescent protein
(EGFP) reporter system in BHK-21 cells. The antiviral potential of the plasmid in BHK-21 cells and suckling mice were investigated.
The results indicate that cotransfection of pCWN11 with any one of three serotypes VP1-EGFP plasmids resulted in a reduction
in the EGFP signal relative to the control. Moreover, the antiviral potential induced by pCWN11 was evident during challenge
with one FMDV isolate of either serotype O (HKN/2002) or serotype Asia I (YNBS/58), and the inhibition extended to almost
40 h. Furthermore, subcutaneous injection of pCWN11 in the neck made suckling mice significantly less susceptible to FMDV
serotype O and Asia I. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|