| 西伯利亚白刺的微繁技术 |
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| 引用本文： | 张启昌,李双福,其其格. 西伯利亚白刺的微繁技术[J]. 东北林业大学学报, 2008, 36(4): 12-13,28 |
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| 作者姓名： | 张启昌 李双福 其其格 |
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| 作者单位： | 北华大学,吉林,132013;吉林省林业厅 |
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| 基金项目： | 吉林省教育厅科学基金项目(200562)，吉林省林业厅科研项目(03012) |
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| 摘 要： | 以西伯利亚白刺(Nitraria sibirica)的幼嫩茎段为外植体,采用正交设计及方差分析对影响西伯利亚白刺植株离体培养的因素进行了优化研究,培育出了西伯利亚白刺的组培苗。结果表明,适合初代培养的培养基是N6 IBA1.0mg/L 6-BA1.0mg/L,适合继代增殖的培养基为N6 IBA0.1mg/L 6-BA2.0mg/L,适合壮苗的培养基是不加激素的N6培养基,适合生根的培养基为MS IBA0.5mg/L。
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| 关 键 词： | 西伯利亚白刺 幼茎 离体培养 激素组合 |
| 收稿时间： | 2007-07-15 |
| 修稿时间： | 2007-07-15 |
Micropropagation of Nitraria sibirica |
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| Zhang Qichang,Li Shuangfu,Qi Qige. Micropropagation of Nitraria sibirica[J]. Journal of Northeast Forestry University, 2008, 36(4): 12-13,28 |
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| Authors: | Zhang Qichang Li Shuangfu Qi Qige |
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| Abstract: | The influencing factors of Nitraria sibirica in vitro cultivation were optimized by orthogonal design and variance analysis using tender stem of N.sibirica as explants.Tissue cultured seedlings were obtained by means of culture medium with different concentrations of hormone.Results show that N6 culture medium supplemented with IBA 1.0 mg/L and 6-BA 1.0 mg/L is the optimal medium for primary culture,N6 with IBA 0.1 mg/L and 6-BA 2.0 mg/L for subculture proliferation,N6 without any hormone for seeding strengthening,and MS with IBA 0.5 mg/L for rooting. |
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| Keywords: | Nitraria sibirica Tender stems Culture in vitro Hormone combination |
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