|
|||||
|
|
| 刺山柑(Capparis spinosa)总RNA不同提取方法的比较 | |
| 引用本文： | 栾东东,石庆华,陈虹,董玉芝.刺山柑(Capparis spinosa)总RNA不同提取方法的比较[J].干旱区研究,2009,26(3). |
| 作者姓名： | 栾东东 石庆华 陈虹 董玉芝 |
| 作者单位： | 1. 新疆农业大学林学院,新疆,乌鲁木齐,830052 2. 新疆农业大学,农业生物技术重点实验室,新疆,乌鲁木齐,830052 3. 新疆农业大学林学院,新疆,乌鲁木齐,830052;新疆农业大学,农业生物技术重点实验室,新疆,乌鲁木齐,830052 |
| 摘 要： | 以野生刺山柑叶片为材料,采用70%丙酮抽提研磨材料,结合TRIZOL,CTAB,SDS 3种常见的RNA提取方法提取总RNA,通过RNA获得率、纯度、电泳图谱以及RT-PCR反应等分析,初步确立了一种有效的提取刺山柑叶片总RNA的改良SDS法.该方法提取的RNA A<,260>/A<,280>值在1.88～1.94之间,电泳图谱完整,具有产量高、时间短、成本低等特点,所得的RNA可以用于RT-PCR和cDNA文库构建以及Northern杂交等后续实验. |
| 关 键 词： | 刺山柑Capparisspinosa RNA提取 CTAB法 SDS法 TRIZOL法 RT-PCR反应 |
Comparison of Different Methods for RNA Extraction from Capparis spinosa |
|
| LUAN Dong-dong,SHI Qing-hua,CHEN Hong,DONG Yu-zhi.Comparison of Different Methods for RNA Extraction from Capparis spinosa[J].Arid Zone Research,2009,26(3). | |
| Authors: | LUAN Dong-dong SHI Qing-hua CHEN Hong DONG Yu-zhi |
| Institution: | 1.College of Forestry;Xinjiang Agriculture University;Urumqi 830052;China;2.Key Laboratory of Agricultural Biotechnology;Agriculture University;China |
| Abstract: | Capparis spinosa is a shrub with high cultivation and economic value in desert and semi-desert areas,and it is a rare species for studying plant stress resistance.An effective method of isolating total RNA from the leaves of wild Capparis spinosa is developed.RNA is extracted by the methods of Trizol regent,CTAB and SDS by combining with 70% acetone.After separately analyzing the rate of yield,purity,quality,electrophoretograms and RT-PCR production,an improved effective SDS method is preliminary developed ... |
| Keywords: | Capparis spinosa RNA extraction CTAB SDS TRIZOL RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |