| 油茶乙酰辅酶A羧化酶BC亚基全长cDNA克隆及序列分析 |
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| 引用本文： | 谭晓风,蒋瑶,王保明,张琳.油茶乙酰辅酶A羧化酶BC亚基全长cDNA克隆及序列分析[J].中南林业科技大学学报,2010,30(2). |
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| 作者姓名： | 谭晓风 蒋瑶 王保明 张琳 |
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| 作者单位： | 谭晓风,张琳(中南林业科技大学,经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004;中南林业科技大学,林学院,湖南,长沙,410004;中南林业科技大学,国家油茶科学中心生物技术实验室,湖南,长沙,410004);蒋瑶,王保明(中南林业科技大学,经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004) |
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| 基金项目： | 国家"十一五"科技支撑项目，国家自然科学基金项目，湖南省自然科学基金项目 |
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| 摘 要： | 以油茶‘湘林1号’品种的近成熟种子为材料,采用简并PCR、RACE、交错PCR等技术,获得了油茶乙酰辅酶A羧化酶BC亚基基因的全长cDNA克隆。该基因cDNA序列全长1 901 bp,含有一个1 599 bp的ORF,编码533个氨基酸残基。BC蛋白的等电点pI为6.88,分子量为58 509.3 u,含两个跨膜结构域,是一个不稳定的非分泌蛋白。模体搜索结果表明在BC上具有N-糖基化位点、蛋白激酶C磷酸化位点、ATP结合位点等多个功能结构域。同源建模的模型中发现12个α-螺旋,整个BC蛋白为一个内凹的结构。该基因已登录到GenBank,并被命名为co-bc。
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| 关 键 词： | 油茶 乙酰辅酶A羧化酶 基因克隆 序列分析 |
Cloning and sequence analysis of BC gene from Camellia oleifera |
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| TAN Xiao-fenga,b,c,JIANG Yaoa,WANG Bao-minga,ZHANG Lina,c.Cloning and sequence analysis of BC gene from Camellia oleifera[J].Journal of Central South Forestry University,2010,30(2). |
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| Authors: | TAN Xiao-fenga b c JIANG Yaoa WANG Bao-minga ZHANG Lina c |
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| Institution: | TAN Xiao-fenga,b,c,JIANG Yaoa,WANG Bao-minga,ZHANG Lina,c(a.The Key Lab of Non-Wood Forest Product of State Forestry Administration,b.School of Forestry,c.The Biotechnology Lab of National Camellia oleifera Science Center,Central South University of Forestry , Technology,Changsha 410004,Hunan,China) |
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| Abstract: | With the near-matured seeds of 'Xianglin No.1' of Camellia oleifera as material to extract total RNA,the whole length cDNA of BC gene that is a subunit of ACCase from Camellia oleifera was obtained by means of the techniques such as degenerate PCR,RACE and the overlap extension PCR.The full-length of cDNA sequence of BC gene is 1 901 bp,and has a 1 599 bp ORF coding 533 amino acids.The pI value of BC protein is 6.88,the molecular mass of the protein is 58 509.3 u,the protein bears two trans-membrane domain,... |
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| Keywords: | BC Camellia oleifera ACCase BC gene cloning sequence analysis |
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