Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Rice Line GD-8S by Molecular Marker-Assisted Selection |
| |
Authors: | LIU Wu-ge # JIN Su-juan ZHU Xiao-yuan WANG Feng LI Jin-hua LIU Zhen-rong LIAO Yi-long ZHU Man-shan HUANG Hui-jun LIU Yi-bai |
| |
Affiliation: | 1. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;2. Agronomy College, Jiangxi Agricultural University, Nanchang 330045, China;3. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; #These authors contributed equally to this paper |
| |
Abstract: | The broad-spectrum blast resistance gene Pi-1, from donor line BL122, was introduced into a thermo-sensitive genic male sterile rice line GD-8S, which possessed good grain quality but high susceptibility to rice blast, by using backcross breeding and molecular marker-assisted selection. Five elite improved male sterile lines, RGD8S-1, RGD8S-2, RGD8S-3, RGD8S-4 and RGD8S-5, were selected based on the results of molecular marker analysis, spikelet sterility, recovery rate of genetic background and agronomic traits. Thirty-three representative blast isolates collected from Guangdong Province,China were used to inoculate the improved lines and the original line GD-8S artificially. The resistance frequencies of the improved lines ranged from 76.47% to 100%, much higher than that of the original line GD-8S (9.09%). On the agronomic characters, there were no significant differences between the improved lines and GD-8S except for flag leaf length and panicle number per plant. The improved lines could be used for breeding hybrid rice with high blast resistance. |
| |
Keywords: | rice blast resistance thermo-sensitive genic male sterile line molecular marker-assisted selection breeding |
本文献已被 CNKI 维普 万方数据 ScienceDirect 等数据库收录! |
| 点击此处可从《水稻科学》浏览原始摘要信息 |
|
点击此处可从《水稻科学》下载全文 |
|