Dietary methionine source and level affect hepatic sulfur amino acid metabolism of broiler breeder hens |
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Authors: | Jianmei Wan Xuemei Ding Jianping Wang Shiping Bai Huanwei Peng Yuheng Luo Zhuowei Su Yue Xuan Keying Zhang |
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Institution: | Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan, China |
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Abstract: | A study was carried out to investigate the effects of dietary methionine source and level on plasma free amino acids patterns and the expression of genes involved in hepatic methionine metabolism in broiler breeders. A total of 2184 broiler breeders were assigned to 13 dietary treatments, with eight replicates per treatment. The 13 treatments included one control group and 12 additional treatments employing two sources and six levels (0.05, 0.10, 0.15, 0.20, 0.25 and 1.00%). Higher plasma methionine concentration was measured for DL‐methionine (DLM) treated hens. Plasma alanine concentration was linearly increased as DLM or 2‐hydroxy‐4‐(methylthio) butanoic acid (HMTBA) supplementation level increased. There was a linear increase in concentrations of tyrosine, valine, glycine and serine as dietary DLM supplementation level increased. Hens treated with DLM had higher relative expression of ADA than those fed HMTBA. The expression of MS, ADA, SAHH and MAT2A changed quadratically as HMTBA supplementation level increased, while the expression of GNMT and SAHH changed quadratically as DLM supplementation level increased. In conclusion, the effects of HMTBA on plasma free amino acid patterns and the expression of hepatic genes involved with methionine are different from DLM. |
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Keywords: | methionine methionine hydroxyl analogue plasma free amino acid sulfur amino acids metabolism |
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