Bi-PASA技术及其在RYR1基因多态性研究中的应用

双向ＰＣＲ扩增特异等位基因是一项建立在ＰＣＲ基础之上的分子生物学新技术。它通过２２对引物的一次ＰＣＲ反应可经迅速、有效地检测出基因组中单个碱基的突变，从面识别个体基因型的差异。现以猪氟烷基因即兰尼定受体基因的多态性检测为例，介绍该技术的基本原理、主要实验步骤以及注意事项。

Bi-PASA technique and its application in the study on the polymorphism of RYR1 gene

Bidirectional PCR amplification of specific alleles(Bi PASA) is a simple method to distinguish single base changes and to genotype homozygotes and heterozygotes in a single PCR reaction.Two alleles are amplified simultaneously and in opposite directions relying on allele specific PCR.The technique requires two pairs of novel primers(two outer primers and two allele specific inner primers) and appropriate cycling conditions.The overview summarized the development and general protocols with an example of RYR1 gene.