小单孢菌E2 菌株对辣椒疫霉的抑制及抗菌物质的初步测定

利用平板稀释法从辣椒疫病病圃根冠土中分离获得一株放线菌E2,根据形态特征和培养性状初步鉴定为小单孢菌Micromonospora sp..用E2菌株的发酵液和菌丝的提取物进行抑菌试验,结果表明,该菌对多种真菌和细菌具有抑菌作用,其含菌发酵液对辣椒苗期人工接种疫霉的防治效果达75%～85%.捷克八溶剂系统纸层析和抗生素特征化学反应表明,E2菌株产生多烯类抗生素.

Control of pepper Phytophthora blight using Micromonospora sp. and identification of the antibiotic substance

A strain of actinomycetes E2,which was isolated from the rhiszosphere of diseased pepper, was primarily identified as Micromonospora sp.based on morphological and cultural traits.A study was carried out on the inhibiting effects of the fermented broth and extracts of E2's mycelia.The results showed that E2 could inhibit the growth of various fungi and bacteria and the inhibition was stable.The control effect of E2 to Phytophthora blight of pepper seedling could reach 75%-85%.By means of antibi- otic special identification technique including the paper chromatography of eight kings of solvent system and characteristic chemical reaction,E2 was also indicated to produce polyene-like substances.