| 南瓜发芽种子DNA提取方法 |
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| 引用本文： | 刘泽发,姜艳芳,孙小武,罗育才,曾永贤. 南瓜发芽种子DNA提取方法[J]. 吉林蔬菜, 2012, 0(4): 64-66 |
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| 作者姓名： | 刘泽发 姜艳芳 孙小武 罗育才 曾永贤 |
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| 作者单位： | [1]湖南人文科技学院,娄底417000 [2]湖南农业大学园艺园林学院,长沙410128 [3]邵东县农业局,湖南省422800 |
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| 基金项目： | 娄底市科技计划项目;湖南省教育厅青年基金项目（11C0708）;湖南人文科技学院青年基金项目（2011QNOS）. |
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| 摘 要： | 实验采用改良的CTAB法成功提取南瓜发芽根尖细胞DNA,其质量和纯度达到与试剂盒提取DNA相当水平,产率高于试剂盒提取DNA的产率。实验证明该方法提取的DNA能够满足分子生物学研究需要,在南瓜杂交种子纯度快速检测应用中具有应用价值。
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| 关 键 词： | DNA提取 南瓜 根尖细胞 改良CTAB法 |
Method Study of Extracting DNA from Watermelon Root Tips |
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| LIU Ze-fa,Jiang Yan-fang,SUN Xiao-wu,LUO Yu-caP ZENG Yong-xian. Method Study of Extracting DNA from Watermelon Root Tips[J]. , 2012, 0(4): 64-66 |
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| Authors: | LIU Ze-fa Jiang Yan-fang SUN Xiao-wu LUO Yu-caP ZENG Yong-xian |
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| Affiliation: | j ( 1.Hunan Universily of Humanities, Science and Technology,Loudi, Hunan,417000; 2.Lan(tiscape and ttorticuhural Institute of Hunan Agriculture Universily, ChangSha, Hunan, 410128 ; 3.Agricuhural Bureau of Shaodong County, Hunan Shaodong, 422800 ) |
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| Abstract: | DNA was extraeled from root tips of pumpkin by a new CTAB method suecesstidly.Extraeting result of new CTAB method was belier than that of method by reagent kit from leaves and its quality and purity was satisfied for experiment.The experiment shows this method has great advantage in hybrid pumpkin seed purity rapid detection. |
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| Keywords: | DNA extraction Pumpkin root tips New CTAB method |
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