黄芩同源四倍体的诱导及细胞形态学观察

在组织培养条件下,把带有绿色芽点的黄芩(Scutellaria Baicalensis Georgi)愈伤组织通过在培养基中添加不同浓度的秋水仙素的培养基上培养的方法,或经0.1%、0.2%、0.3%秋水仙素溶液浸泡一定时间后再进行培养,均可诱发黄芩多倍体的产生,前者以20 mg/L的秋水仙素浓度诱导效果最好,诱导率可达20%,而后者以0.2%秋水仙素溶液浸泡12 h效果较好,诱导率可达43.3%.

The Inducing of Autotetraploid of Scutellaria baicalensis Georgi

In tissue culture conditions,autotetraploid of S.baicalensis Georgi can be induced by adding colchicine in medium,or by immersing the callus of S.baicalensis Georgi in 0.1%,0.2%,0.3% colchicine solution for a period of time prior to culture.The former was that 20 mg/L concentration of colchicine induced by the best,with an inducing ratio of 20%.And the latter was that 0.2% colchicine solution soaking 12 h was better-performing,with an inducing ratio of 43.3%.