Determination of 2,6-diaminopimelic acid in swine feces and chymus |
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| Authors: | T V?lker J Wünsche E Borgmann W B Souffrant |
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| Affiliation: | Oskar-Kellner-Institut für Tierern?hrung Rostock. |
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| Abstract: | A method is described for estimation of 2.6-diaminopimelic acid (DAP) using an automated amino acid analyzer and MOORE and STEIN ninhydrin reagent. Investigations were made concerning the influence of sample treatment on DAP content of faeces and ileal digesta of pigs and isolated bacteria. Oxidation before hydrolysis did not change DAP content of faeces and bacterial samples, but increased DAP of digesta. Since analytical reasons were excluded, different accessibility of DAP in faeces and digesta for hydrolysis is suggested. Lyophilization or preservation of fresh samples with formaldehyde and phenolic solution, resp., resulted in no significant influence on DAP content. |
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