Sodium azide induced high-oleic peanut (Arachis hypogaea L.) mutant of Virginia type |
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Authors: | Nkuna Rivalani Theorent Wang Chuan Tang Wang Xiu Zhen Tang Yue Yi Wang Zhi Wei Zhang Jian Cheng |
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Institution: | 1.Jilin Agricultural University., Changchun, 130118, China ;2.Shandong Luhua Group Company, Laiyang, 265200, China ;3.Shandong Peanut Research Institute, Qingdao, 266100, China ; |
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Abstract: | Less prone to oxidation than its conventional counterpart, high-oleic peanut (Arachis hypogaea L.) containing?≥?72% oleate and?<?8% linoleate is preferred by processors, seed sellers and consumers. Development of high-oleic peanut cultivars with suitable pod/seed size and shape may satisfy the need from food processors manufacturing whole kernel or halve products. In this study, a high-oleic Virginia type peanut mutant was identified through screening of a 15 mmol/L sodium azide mutagenized M3 population with near infrared spectroscopy. Sequencing of the mutated and wild type FAD2A/FAD2B genes detected 2 point mutations. The G448A mutation in FAD2A was the same as in previous reports, causing an amino acid change of D150N. G558A in FAD2B was a novel mutation, resulting in a stop codon and premature termination of protein synthesis. 16 promising lines with acceptable productivity and pod/seed characters have been tentatively bred, which will be evaluated further in yield tests with replications after seed increase. |
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